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10.1002/bit.260261012.abs Dihydrofolate reductase, purified to homogeneity from amethopterin‐resistant Lactobacillus casei, was immobilized by coupling to cyanogen bromide‐activated Sepharose or carbodiimide‐activated CH‐Sepharose. Coupling yields were determined by amino acid analysis...
10.1002/bit.260261002.abs An inducible β‐galactosidase from an extremely thermophilic organism, Thermus strain 4−1A, has been isolated and partially purified. There were significant dissimilarities to T. aquaticus β‐galactosidase. It had a pl of 4.5, was inhibited by sulphydryl inhibitors...
10.1002/bit.260261014.abs A novel algorithm for predicting BOD5 from the dissolved oxygen (DO) concentration after a relatively short incubation period is presented and evaluated experimentally. Test runs on synthetic and experimentally derived raw data suggest that BOD5 can be predicted to...
10.1002/bit.260261006.abs An integrated approach is presented for the on‐line estimation of the state of a biochemical reactor from presently attainable real‐time measurements. Elemental and macroscopic balances are used for the determination of the total rate of growth and...
10.1002/bit.260261011.abs After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene–divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were...
10.1002/bit.260261010.abs Lignocellulose materials were pretreated by methods known to improve enzymatic saccharification, and the percentage crystallinity (x‐ray diffraction) and degree of polymerization were measured. It was observed that although the percentage crystallinity of cellulose was...
10.1002/bit.260261016.abs Wheat straw (Ws)was treated in a pilot‐scale continuous mixer to disrupt the lignin–hemicellulose–cellulose (LHC) complex. An efficient and practical method was desired to remove lignin and hemicellulose (pentosans)rapidly and efficiently from the lignocellulose...
10.1002/bit.260261008.abs The applicability of the respiratory quotient measurement, a heat evolution measurement, and a commonly observed correlation between the respiratory quotient and product yield to on‐line bioreactor identification and control were inspected. It was found that...
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