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10.1002/bit.260220211.abs The hydrophobic interaction of β‐galactosidase with Sepharose 4B substituted with 3,3′‐diaminodipropylamine was studied in both batch and column experiments. The equilibrium and the binding rate constants were determined for different phosphate buffer...
10.1002/bit.260220210.abs White‐rot fungi, which have the ability to degrade all the wood components including lignin, are of great interest in biotechnological processes based on wood and other lignocellulosic materials. It was demonstrated earlier that enough lignin can be degraded to cause a...
10.1002/bit.260220207.abs Enzyme stability studies have been reinvestigated under the conditions used for cellulose hydrolysis (pH 4.8, 50°C, 24 hr). The cellobiohydrolase (CBH) component as measured on Avicel is less stable than other enzymes of the cellulase complex, and is 60% inactivated by...
10.1002/bit.260220203.abs Two types of polyethylenimine‐coated glass microbeads (13–44 μm) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their...
10.1002/bit.260220212.abs The growth kinetics of Bacillus subtilis KYA 741, an adenine‐requiring strain, was investigated under adenine‐limiting conditions. The concentration of adenine (the limiting substrate for cell growth) in the culture filtrate remained constant during the stationary...
10.1002/bit.260220214.abs Candida lipolytica was cultured batchwise using n‐hexadecane as the main carbon source. Biomass production, n‐hexadecane consumption, oxygen consumption, and carbon dioxide evolution were measured to follow the fermentation. The consistency of the measured data was...
10.1002/bit.260220204.abs Using continuous and fed‐batch penicillin fermentation systems some important metabolic parameters have been determined for the purpose of achieving process improvement and better process control. The specific uptake rates determined under the optimal conditions are:...
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