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Immobilization of an enzyme within an insoluble material permeable to substrate can change the apparent behavior of the enzyme. In particular, external mass transfer and intraparticle diffusion effects can significantly influence the dependence of observed reaction rate on operating parameters...
An agarose gel modified with N‐(ω‐aminohexytl)‐L‐aspartic acid (AHA) and spiropyran compound (AHA–spiropyran gel) was prepared and the photocontrolled binding and releasing of asparaginase were investigated with the AHA–spiropyran gel. Asparaginase was bound on the AHA–spiropyran...
Mathematical models presented in a previous paper (Biotechnol. Bioeng., 20, 865 (1978)) describing unsteady‐state bacterial growth on organic polymers are modified to predict the holding time necessary to attain a given treatment level in a steady‐state activated sludge process. Various...
The shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the...
Modeling of microbial growth using nonmiscible substrate is studied when kinetics of substrate dissolution is rate limiting. When the substrate concentration is low, the growth rate is described by an analytical relation that can be identified as a Contois relationship. If the substrate...
Solid‐state fermentations (78% initial moisture content) of alkali‐pretreated Eastern Hard Maple sawdust were conducted in tray and tumble fermentors using chaetomium cellulolyticum. Crude protein content of the solids rose from 0.9 to 11% in the tray fermentor and 8% in the tumble fermentor...
Candida lipolytica was grown continuously on n‐hexadecane as the main source of carbon. A transient continuous‐culture experiment was also conducted to investigate hydrocarbon‐limited growth; the hydrocarbon feed flow rate was stopped for several hours and then resumed at a reduced...
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