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A two‐stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The...
Continuous, steady‐state grape‐juice fermentation, carried out in experimental slant‐tube fermentors, was characterized by complete fermentation, even at fast pumping rates, because efficient cell retention, as well as rapid yeast growth, quickly built cell density up to the limit permitted...
Experiments were carried out on dextran–dextranase systems to test the prediction of a mechanistic model recently proposed by us, for the synergistic effect of combined exo/endo enzymic action in the degradation of polymeric substrate. Soluble forms of the substrate were used. Preliminary...
Glucose isomerase in the form of heat‐treated whole‐cell enzyme prepared from Streptomyces phaeochromogenus follows the reversible single‐substrate reaction kinetics in isomerization of glucose to fructose. Based on the Kinetic constants determined and the mathematical model of the reactor...
Pressure drop, gas hold‐up, and oxygen transfer were investigated in a sieve tray column, a column with Koch motionless mixers, and a bubble column. The oxygen transfer experiments were conducted using cocurrent flow of gas and liquid under steady‐state conditions with oxygen transfer from...
The kinetic behavior of a system of multiple enzyme in solution has been studied in a variable volume batch reactor at pH 5, controlled dissolved oxygen concentration, and T = 30°C. The enzymes used were glucoamylase (R. delemar), glucose oxidase (A. niger), and gluconolactonase (A. niger), all...
The properties of intracellular RNase in disintegrated cell suspensions of Saccharomyces cerevisiae have been studied. The influence of salt addition and/or incubation of the suspension on the activity of RNase and on the degradation of endogenous RNA was determined. No significant change in the...
A new mechanochemical method for enzyme immobilization has been elaborated. The principle of this method consists of the following precepts. Partially hydrolyzed nylon fiber, the surface of which is known to be strewn with micro‐cracks, is reversibly stretched (∼25%) and placed into an enzyme...
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