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Lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide and an intermediary product is formed with antibacterial properties. The components of this system, with the exception of hydrogen peroxide, are present in milk. H2O2 may be introduced by means of enzymatic, generation...
Reactor systems based on tapered fluidized beds are being developed for aqueous bioprocesses in which adhering microorganisms or immobilized active biological fractions are used. The use of a fluidized bed prevents biomass buildup, accommodates particulates in the feed stream, is compatible with...
The enzyme tyrosinase (E.G. 126.96.36.199) has been immobilized in a polyacrylamide gel and intermittently assayed for enzyme activity over a period of 19 days using phenol as the substrate. The results of these studies indicate that the immobilized enzyme could be incorporated into a system to...
We have demonstrated the use of bacterial chromatophores for the continuous photosynthetic regeneration of ATP from ADP in an ultrafiltration reactor. Biphasic kinetics of the degradation of chromatophore activity are described. Using chromatophores in combination with the enzyme adenylate...
A method for calculating the mass transfer coefficient in a biological film system, under turbulent flow conditions, is presented. It is experimentally found that fluid velocity has a positive effect on the rate of substrate utilization when the system is operated in other than the kinetic...
Feather meal protein was prepared in granular form and used as a support for lactase using glutaraldehyde as the crosslinking agent. The support gave a high retention of activity and in column operation it yielded apparent half‐lives from 50 to 100 days. Because of its gel‐like consistency...
The construction and use of an automated membrane filtration system is described which produces 300 liters per day of sterile medium over at least four months of continuous fermentation. The capacity of the system is limited only by choice of filter type and has applications in continuous pilot...
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