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Little is known about techniques for applying untreated microbial cells containing enzymes directly to industrial processes as a biocatalyst. The kinetic behavior of α‐galactosidase‐containing spherical pellets which are formed naturally under given conditions in a submerged culture of...
A well‐mixed biological reactor with continuous addition of biological solids was subjected to sinusoidal variations in inlet substrate concentration. The phase lag between inlet and outlet concentrations increased with increasing frequency. The deviation of measured substrate concentrations...
Lactose (β‐galactosidase) was attached to the inner surface of nylon tubing. Tubes of various lengths were used to bring about the hydrolysis of o‐nitrophenyl‐β‐D‐galactoside and of lactose in skim milk. The results with the former substrate were analyzed in the light of a theoretical...
The apparent activation energy of N‐α‐benzoyl‐L‐arginine‐ethyl ester (BAEE) hydrolysis by immobilized trypsin varies with the bulk substrate concentration from its maximum value, comparable to that of the free enzyme, to considerably lower values. Thus, with a concentration change from...
Escherichia coli B, Escherichia coli MRE 600, Escherichia coli K 12‐3300, Pseudomonas fluorescens, and Aerobacter aerogenes were grown exponentially in a bench‐scale fermentor to cell concentrations in the range of 20 to 41 g dry cells/liter at 30°C and 30 to 55 g dry cells/liter at 25°C....
Trypsin was covalently immobilized on porous glass in the presence and absence of a specific substrate and reacted in various organic solvents of different dielectric constants. Optimum solvent concentration, pH profile, Km(app), Vmax(app), productivity versus temperature, activity, and reaction...
The criterion for the oxygen limitation of substrate uptake in microbial film fermenters is expressed in terms of diffusion coefficients, utilization coefficients, and the free solution concentrations of substrate and oxygen. It is proposed that the ideal film thickness in such fermenters is...
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