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The formation of gibberellic acid in single‐stage continuous cultures was studied. Stirred fermenters of 3 and 20‐l. working volumes were used. Maximum output of gibberellic acid was obtained at a holding time of 200 hr. The rate of formation, calculated on the basis of mycelial nitrogen, was...
The construction of a “Porton type” chemostat of 8‐l. working volume, based on the New Brunswick Scientific Co., Inc., Model F‐14 Fermentor, is detailed. Instrumentation for temperature, pH, and foam control, and for medium supply are described and operational problems discussed.
A process has been developed whereby arginine decarboxy‐oxidase (ADO) can be produced by growing Streptomyces griseus in submerged form in 20‐l. and 200‐l. tank fermentors. A medium containing salts, corn steep, glucose, or starch is used. With addition of 0.02% arginine and with limited...
The oxygen demand of Penicillium chrysogenum culture was supplied by an automatic aeration control system on the basis of the actual respiration of the cells. The simultaneous use of the automatic aeration apparatus and a turbomixer of great shear stress revealed that some effects of agitation...
Evidence for generation of the plateau in oxygen uptake during exertion of carbonaceous BOD by pure cultures was sought by making long term Warburg studies using glucose and phthalic acid as substrates. One organism, tentatively identified as Escherichia intermedia, of the 57 tested, exhibited a...
A laboratory process for the isolation of acid soluble nucleotides from bacteria has been scaled‐up. Escherichia coli was grown in 220‐l. batches, harvested by centrifugation, and extracted with cold trichloracetic acid. The nucleotides in the extract were separated by adsorption onto a...
An exploratory study is described on the use of a biphasic culture technique for production of toxin by C. tetani. The biphasic system used was a combination of dextran and poly(ethylene glycol) (PEG) in the presence of a protein‐free medium. The system supported good growth and gave a...
Many antimicrobial agents are available for commercial use, but only a few are truly sporicidal and can be used as chemosterilizers. The action of ethylene oxide, propylene oxide, and β‐propiolactone is well documented. Strong acids, e.g., hydrochloric acid, or alkalis may be used as...
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