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Fern gametophytes were grown in liquid medium on the surface of plastic tissue culture flasks (Falcon Plastics) where they remained attached through radioactive precursor incorporation, fixation in buffered 3% glutaraldehyde, postfixation in buffered 2% OsO 4 , alcoholic dehydration, and...
To overcome the difficulty of obtaining compact slices or pieces of tissue from the rat testis, due to the scarcity of interstitial tissue, the fixation of the testis is started in the live anesthetized animal by injecting 3% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.2 under the...
Blood smears from normal and reserpinized rabbits were dried in a desiccator and treated with gaseous formaldehyde. The normal blood smears showed an intense yellow fluorescence due to serotonin in platelets. Fluorescence was weak or absent in the smears of the reserpinized rabbits. A standard...
Endospore nuclei of Protomyces inundatus may be stained satisfactorily with either acetic orcein or Giemsa after the following treatment: 1.5% sodium acetate, 12 hrs, 1:3 acetic alcohol 2–4 days, wash in absolute alcohol, transfer through 1:1 alcohol-chloroform to chloroform for 30 min and...
Mounting of support films and sections for transmission electron microscopy has been examined with the scanning electron microscope. Experiments have been designed to test the adherence of support films to polished and matte surfaces of specimen grids. It is the conclusion of the authors that...
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