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The number and shape of pollen nuclei in cytologically refractive plants can be determined from anthers fixed in FAA, preserved in 70% ethanol, treated 10–30 min with 50% HCl in 95% ethanol, and stained with iron acetocarmine saturated with chloral hydrate, 1–16 hr. Pollen is then pressed...
The heterogeneity of bromophenol blue from different commercial sources was revealed by paper chromatography. Isopropanol:ammonia:water (20:1:2) as the solvent system gave the best separation. A variety of impurities: violet, pink, light blue and yellow coloured ones were observed. Two of the...
Eleven decalcifying fluids were tested for rate of decalcification and for their effect on subsequent staining by each of 4 variations of the Alcian blue technique. Tests for decalcification were made on 5 mm slices of fixed adult rat heads. Tests on staining were made on 5 μm sections cut after...
An improved method is described in which tissue areas can be initially identified in thick sections by light microscopy and isolated for subsequent ultrathin sections and observation by electron microscopy. This is achieved by embedding in hard Epon which can be sectioned at 25–150 μm on a...
A mixture consisting of 1% phosphotungstic acid (PTA) in 10% chromic acid (CrO 3 ) selectively stains the plasma membrane of plant cells. Whole tissue or pelleted cell fractions are prepared for electron microscopy using conventional methods including glutaraldehyde fixation and OsO 4...
Various stains were tested with suspension cell cultures of Nicotiana tabacum (tobacco), Oryza sativa (rice), Glycine max (soybean), Daucus carota (carrot) and Lycopersicon esculentum (tomato). A drop of the culture medium containing each dye was mixed with a drop of cells on a microscope slide...
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