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The epoxy resin was removed from semithin (1 μm) sections by immersing them for 30 sec in sodium methoxide (Mayor et al. , J. Biophys. Biochem. Cytol., 9: 909–10, 1961) and then processed as follows: (1) left for 1–3 hr at 60 C in a mixture of formalin, 25 ml; glacial acetic acid, 5 ml; CrO...
Metaphase chromosomes of humans and other species can be identified by their quinacrine fluorescence patterns. For the study of human chromosomes, standard phytohemagglutinin-stimulated leukocytes are used. A 45–60 min interval of colchicine treatment is used to obtain a higher proportion of...
By introducing variations in procedures and comparing density and patterns of degeneration exhibited, we found that: (1) in the rat, cat, and monkey, the optimum survival time was about 1 wk; this period yielding the maximum amount of argyrophilic reaction and the most consistent staining among...
Three simultaneous azo dye coupling reactions and an indigogenic phosphate reaction were compared for their ability to detect chicken intestinal alkaline phosphatases separated in polyacrylamide disc gels. The diazoniums examined, Fast Red TR, Fast Blue RR and Nuclear Fast Red Salt, used sodium...
Developing and established nerve fibers in the retina and in superficial tracts of the brain can be stained and viewed en bloc. The method was developed on chick embryos of 2 days of incubation to several months post-hatching but could be used on other material provided that the objects of...
Slices of fetuses dissected by the Wilson free-hand razor blade slicing method can be stained by the following procedure: Place washed slices (Bouin-fixed, stored in 80% alcohol) in staining solution made fresh daily by diluting 1:20 a stock aqueous solution of 0.1% cresyl violet acetate with 2%...
Commonly used physical and chemical methods for relaxing digenea and other helminths were applied to Fasciola hepatica before fixation. These methods were found to give no better preparations than direct use of fixatives applied at 70–90 C. Neither the shape of the whole mount, nor its...
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