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The Golgi apparatus can be demonstrated in frozen sections of various tissues with an aqueous solution of 1% KMnO 4 . The externum of Golgi apparatus can be stained with 0.05 gm of benzidine in 10% acetic acid. The internum of Golgi apparatus can be stained with 1 gm of basic fuchsin in 99 ml of...
This is an adaptation of procedures used to prepare autoradiographs for electron microscopy to light microscopy. A rectangular wire loop slightly larger than the slide is used. The liquid emulsion is prepared by mixing 1 volume of a 0.1% solution of Dreft or Ivory Snow with 2 volumes of Kodak...
Full term rat foetuses were skinned and eviscerated prior to fixation in 10% formol-saline. The bodies were then stained with 0.05% methylene blue in 17.5% aqueous ethanol. After preliminary immersion in 1% HC1 in 70% ethanol, differentiation was completed in 70% ethanol. Specimens were...
Frozen sections of formalin-fixed brains are impregnated according to the Nauta-Gygax method. After treatment in a 1% Na 2 S 2 O 3 solution, the sections are washed thoroughly, then bleached for 10–20 sec in a solution prepared by diluting 1 part of a stock solution consisting of: borax, 2 gm;...
Aqueous solutions of the arylmethane dyes Chromoxane pure blue BLD (C.I. No. 43825) and Chromoxane pure blue B (C.I. No. 43830) will stain beryllium oxide. In the presence of EDTA the staining of other metals is masked. As a specific stain for BeO, formol saline fixed paraffin sections are...
Complete blocking of the Schiff reaction applied after HIO 4 oxidation is attained by 1%, 0.5% or 0.2% NaBH 4 in 1% Na 2 HPO 4 with a 1 min exposure, 0.1% NaBH 4 requires 2 min. KBH 4 was also completely effective in the same solvent at 1, 0.5 and 0.25% in 1 min. The solutions deteriorate on...
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