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Aqueous 0.1% neutral red adjusted to pH 6.5, or mixed 1:1 with seawater, produced conspicuous fluorescence in plant and animal fats while acting as a conventional stain for acidic groups including volutin. The water-soluble, nonfluorescent dye predominating in acid solutions became deprotonized,...
Air-dried pollen of Tradescantia paludosa and a colchicine doubled Allium ascalonium-fistulosum interspecific hybrid was sown in culture tubes on an inorganic salt-lactose liquid medium containing 0.02% colchicine. After a 16–20 hr incubation at 22 C, pollen tubes were harvested by...
Electron microscopy can demonstrate Fe or Mn accumulated by living Elodea leaves from a solution containing ions of either of these two metals. The Fe and Mn accumulation is deemed to be metabolic because it does not interfere with growth or cyclosis, and because it occurs in light only–not in...
Aldehyde fuchsin, pseudoisocyanin and toluidine blue, histochemical dyes reported to be specific for insulin–containing granules of the pancreatic beta cell, were applied to insulin fixed in polyacrylamide gel by disc electrophoresis. Two major and four minor bands were resolved as demonstrated...
Fibrin clots were used for suspensions; fibrin films for preserving in situ characteristics of conidial chains, delicate fruiting structures, and cellular arrangements of mycetozoa and fungi. A solution containing fibrinogen, 300–350 mg; Na-citrate, 160 mg; and NaCl, 850 mg per 100 ml, was...
Procedure :Cut paraffin sections and float on a 45–50 C water bath; spread silicone-rubber adhesive (Clear Seal-General Electric) thinly and evenly over 2/3 of the slide; pick up the sections from the floatation water with the coated slide; dry for 1.5 hr at 25 C and at 60 C for 0.5 hr;...
Sections stained with haematoxylin and eosin showed maximum optical density (OD) at 536 nm, with a second peak at 600 nm. Sections stained with only eosin showed a peak at 536 nm, whilst those stained with haetnatoxylin showed a peak at 600 nm. Reduction in OD at these wavelengths was used to...
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