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Samples of Nile blue sulphate (Geigy and G. T. Gurr) were separated by silicic acid thin layer chromatography. The blue sulphated oxazine base was found to consist of 3 components with identical absorption spectra in the visible range. These probably represent isomeric forms of the dye. The red...
Investigations of hyaline deposits in arteriosclerotic lesions disclosed striking differences in the amount of fibrin demonstrated by these two stains. To test their specificity, model slides carrying dried 10% solutions or suspensions of polysaccharides, fractions of plasma and other proteins...
Representative extracted lipids were examined visually and spectrophoto-metrically following staining under varying conditions with the oxazine dye Nile blue sulphate. Optimum results in staining both extracted and tissue lipids were obtained with 1% acid-hydrolysed Nile blue prepared by boiling...
HeLa cells were stained with a 1/12,000 concentration of acridine orange at pH 7.2 for 3 min and the fluorescence emission was measured quantitatively for effects of ultraviolet illumination with durations including intervals between 5 and 210 min. The total photometric fluorescence intensity...
Well-spread metaphase plates for routine karyotype analysis can be obtained by treating the very young leaf-buds of tea shoots in a saturated aqueous solution of p -dichlorobenzene for 2–3 hr at 4–10 C, fixing in a 1:3:6 mixture of propionic acid, chloroform and ethanol for 6–12 hr,...
Staining of tissue sections by basic dyes after immersion in hot hydrochloric acid (0.2 N for 3–10 hr at 60 C) provides a means for selective detection of many endocrine cells. The acid hydrolysis suppresses diffuse basophilia, mainly due to RNA, DNA and acid polysaccharides, and increases the...
Tissue slices not more than 4 mm thick—including those containing mineralized bone—were fixed in buffered 6.25% glutaraldehyde. Parts of the specimen to be used for subsequent electron microscopy had to be within 1 mm of the surface to permit proper penetration of the second fixation in 1%...
Broth cultures of Escherichia coli , strains Hfr G6 and F − 464 grown separately, were mixed (2 ml of each) and the mixture filtered through a 0.45 μ pore size, 25 mm diameter, MF-Millipore membrane. The membrane was placed, cell side up, on a nutrient agar plate and incubated 15 min at 37 C....
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