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Vital staining of aortas from mice injected subcutaneously (daily for 5 days) with trypan blue was studied. In routine paraffin sections elastic membranes were observed to be well stained and other medial elements unstained following fixation in 10% formaldehyde (25% formalin) at pH 7–9. An...
For the study of peripheral nerve fibers and endings, frozen sections of material fixed for 1 wk to 2 mo in Richardson's formalin, (prepared from paraformaldehyde and containing 3.4% sucrose) are extracted in saturated boric acid for 1–18 hr at 37° C, or in formol-alcohol (4 parts absolute...
Small pieces of formalin-fixed tissue are infiltrated first with a 1% and then with a 2% solution of a low viscosity sodium alginate (a salt of a polymannuronic acid obtained from seaweed). This tissue is then transferred to a solution of a high molecular weight sodium alginate containing...
For the meiotic study of Ipomea spp. , flower buds were stripped of the calyx and corolla and soaked in saturated aqueous paradichlorobenzene at about 28° C for 3 hr, transferred to acetic-alcohol (1:3) for 6 hr, then into 1% saponin solution and left overnight. They were mordanted in 1:3...
The floating-out technic, popular for single paraffin sections, can be applied successfully to ribbons of serials by either of two procedures. (1) If spreading time for the sections is uncritical suitable lengths of ribbon for attachment to a slide are laid on water at a temperature about 8° C...
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