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To a 1.0% filtered aqueous solution of toluidin blue add drop by drop 4–5 ml of either a saturated aqueous solution of HgCl 2 or of KI. Collect the resulting dark precipitate on a filter paper and wash it with numerous small quantities of distilled water applied to both inside and outside of...
A sheet of cellulose acetate about 0.01 inch thick is clamped over a mold, heated to softness by an electric heater and drawn down over the mold by means of a vacuum. When cooled, the sheet, now formed into embedding boxes, is removed from the clamp. Boxes so made are inexpensive enough to be...
The Dyer, Chance, alcian blue, and tannic acid-crystal violet wall stains for bacteria were compared. All gave apparent cell wall widths which were considerably greater than those demonstrated by electron microscope methods. The exaggerated width, as seen by the light microscope, was apparently...
A simplified technic for preparation of the aceto-orcein stain permits the storage of cells in the stain or squash preparations at room temperature for long periods without in* jury to or distortion of the cells and mitotic plates. Fresh cells from tumor ascites, tissue culture cells growing in...
Frozen sections, 25-50 /j. thick, of formalin-fixed nervous tissues are mounted following the Albrecht gelatin technic. Paraffin sections, 15 p. , are deparaffinized and transferred to absolute ethanol. The slides are then coated with celloidin. Both frozen and paraffin sections subsequently...
Sudan black B was found to be decomposed in acid solutions. This decomposition was rapid below pH 4. Above this pH the decomposition was markedly slower. After mild acid hydrolysis, the product was studied chromatographically and found to contain a basic dye with a pK of about 4 , a brown color...
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