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A mounted paraffin section of material fixed in Bouin's, Carnoy's or 10% formalin is allowed to stand 15 minutes at room temperature in a 0.3% solution of 8-hydroxyquinoline in 30% ethanol. The slide, with adhering solution, is placed in 0.15 N hypochlorite (with enough KOH added to make the...
The effect of oxidation on neurofibrillar argyrophilia was studied by subjecting nervous tissues containing both normal and degenerating fibers to the action of potassium permanganate, periodic acid, chromic acid, lead tetraacetate, and sodium bismuthate prior to silver impregnation. The...
Soaking paraffin-embedded plant specimens 2–3 days at 37°C. in a mixture of glycerol, 10 ml., Dreft, 1 g., and water 90 ml. is an effective means of softening them prior to sectioning. One side of the paraffin block must be pared away to expose the tissue before immersion in the softening...
A technic is described for producing critically stained preparations of phloem tissue. The preparations promise to be relatively stable. Sections of fixed unembedded or of embedded (paraffin or celloidin) phloem, cambium, and xylem are (1) stained in Foster's tannic acid-ferric chloride...
A comparison of the utility of gallocyanin, gallamine blue, celestine blue B and acid alizarin blue BB as nuclear stains for material to be counterstained by the van Gieson, Kornhauser, Gomori and Masson technics has been made. With all these nuclear stains except gallocyanin there were definite...
The ciliates are manipulated at all stages by means of micropipettes and fine needles, proceeding by the following steps: Fix the material in a small receptacle with Champy's fluid 1–3 minutes following with Da Fano's solution for several hours. Transfer the specimens to a slide, withdraw...
Several factors influencing the staining of nerve fibers with methylene blue, especially the influence of chloralhydrate and carbamylcholine chloride (as parasympathicotonics), and of some anesthetics were studied. The intestines of mouse, rat, and guinea pig were used. The following immersion...
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