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The method of staining involves the principle of reduction and oxidation of some cell component. The reduction is a function of the mordant and the oxidation that of the stain. Three areas of the cells may be differentiated depending upon the condition of the mordant. The wall may remain...
The preparation of tissues by the freezing-drying technic is preferred for many histochemical studies because of the rapid fixation, avoidance of deleterious action of chemical fixation and extraction by aqueous and lipid solvents in fixation, dehydration and clearing; to facilitate this...
“Viscol”, a water soluble permanent mounting medium hardening through evaporation of water under the cover glass, has been analyzed. It proves to consist of a mixture of gum arabic, glycerol, phenol and water and is especially suitable for simple botanical preparations. The use of gum arabic...
Solutions of Parlodion stored in clear glass bottles, and subjected to the usual lighting of laboratory work tables, deteriorate progressively. Blocks of nervous tissue stained by Cajal's formula No. 6 underwent marked fading when embedded in old, light-affected Parlodion solutions. Storage of...
A staining technique for demonstrating reticulin, elastin and collagen in the same tissue sections is based upon the use of a silver stain for reticulin, orcein for elastin and picro-anilin blue or fast green for collagen and other tissue structures.
A satisfactory histochemical test for polyphenols in fresh plant tissues is described. The test is based upon a colorimetric method for phenolics using a nitrous acid reaction. Certain catechol derivatives are characterized by the formation of an intense cherry-red in fresh sections of plant...
A modification of the Golgi technic is described in which the reaction takes place in well fixed formalin material. Thin slices (whole sections of adult monkey, cat and rat cerebrum) 2 to 3 mm. thick, from brains fixed 3 to 4 months in 10% formalin, are chromated for two days in 3 g. of zinc...
A method for softening refractory paraffin-embedded specimens by trimming the block to just expose the tissue and soaking in solutions of wetting agents (detergents) is recommended. Paraffin blocks are soaked several hours to overnight in 1% aqueous solutions of either new Dreft, sodium lauryl...
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