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Supravital staining by thiazins of segments of small intestine and mesentery of young dogs was studied with reference to specificity for nervous tissue. Attempts to secure a purer form of methylene blue by alumina adsorption and alcohol elution of the commercial, medicinal dye yielded a product...
Deterioration of Schiff's (Feulgen's) reagent was studied under varying conditions. Photometric readings were taken of both induced and spontaneous color development and curves which showed the rate of deterioration were plotted. It was concluded that Schiff's reagent, when kept in well-filled...
While acid dyes have not been widely used for staining bacteria, several suggestions for their use have been advanced (Conn and Holmes, 1926; Maneval, 1941). In general these procedures call for adding an acid to the stain to intensify the combination of the dye with the bacterial cell. McCalla...
Gomori's methenamine silver method for argentaffin cells has been modified and considerably accelerated by almost doubling the silver concentration and raising the incubation temperature to 60°C. Argentaffin cells are selectively impregnated in 3 to 4 hours and the background remains relatively...
Stains are listed which have proved suitable for staining the epidermis, mesophyll, and sclerenchyma and tracheary elements, respectively, of cleared leaf material of Mouriri and Linociera. Too rapid leaching is avoided by overstaining high in the dehydration series, destaining briefly in the...
-Results with phase microscopy can often be improved by the proper selection of mounting media. Temporary mounts can first be made in liquid mixtures of water and glycerol, butyl carbitol and alpha-chloronaphthalene, alpha-chloronaphthalene and alpha-bromonaphthalene or alpha-bromonaphthalene...
A method for making permanent whole mounts of flat and round worms is described. The specimens are mounted in a drop of acid fuchsin lacto-phenol on a slide and warmed for 6 hours at 60°C. The acid fuchsin is replaced by light cotton-blue (anilin blue, W. S.) in lacto-phenol, till the desired...
A method is described for the demonstration of a new nerve-cell organoid— the binary spheroid systems (Baker bodies). Zenker-formol or acetic-osmium-bichromate materials are postchromed at 37°C. and embedded in paraffin, sectioned and mounted in the ordinary manner. The sections are colored in...
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