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An improved technic for staining flagella of anaerobic bacilli is described. This involves the use of the flagella stain described by Bailey, and careful attention to several details. Those considered of most importance are listed, as follows: 1. At least two rapid subcultures were made in broth...
Maximow's fixative is doubtless one of the best of those containing osmic acid, as it does not reduce the staining properties and enables the application of a variety of stains. It gives very good results with blood stains, and this is of considerable importance for invertebrate work, where...
The actual mechanism of the differentiation of lactose-fermenting and non-lactose-fermenting organisms on eosin-methylene-blue medium is not reported in the literature. The present study is an attempt to elucidate this problem. The color of colon forms on E.M.B. agar was found to depend on two...
It is shown that by the proper use of a modern dark-field condenser, it is possible to eliminate the difficulties ordinarily encountered in the examination of stained blood smears under darkfield illumination. For hematological studies the proposed method has the following advantages over the...
A microchemical test for cellulose applicable to fresh sections and commercial products is described. The test differs from the older technics in that materials tested are not permanently altered. Two solutions are required: (1) 2% solution of iodine in 5% KI, diluted with 9 parts by volume of...
The metachromatic granules, which sometimes are regarded as characteristic of the diphtherial species, are more easily differentiated when the basic dye is made up in a solution of which the reaction is quite acid. Of the usual stains employed for demonstrating the granules, the Neisser stain...
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