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The isoelectric point of a bacterial system is the hydrogen-ion concentration at which there is equal retention of anion and cation. Defining this point as that at which there is equal retention of acidic and basic stain when acetone is used as a decolorizer, it is shown that acidic decolorizers...
The writers discuss a series of investigations as to the behavior of certain fluorescein dyes (eosin, erythrosin, phloxine, and rose bengal) in staining bacteria in dried films of soil. These dyes are ordinarily purchased in the form of di-sodium salts and are indifferent staining agents for the...
The general parallelism between the Gram reaction and normal selective bacteriostasis by the triphenylmethane dyes is well established, as is also the existence of a small number of organisms in each group which do not follow the rule. Reverse extrinsic bacteriostasis has been demonstrated, but...
Decolorizers which are distinctly acidic or basic in their chemical nature give abnormally high decolorization in the Gram stain for bacteria. Acidic substances yield more regular results. Ideally an “inert” decolorizer should be used, but ordinarily such substances will not dissolve the dye...
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