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Highlights ► We report a functional protease assay using liquid crystals as a signal reporter. ► The results of this protease assay can be observed by naked eye. ► Active and inactive proteases can be differentiated by using this protease assay. ► Both α-chymotrypsin and trypsin can be...
Highlights ► We constructed an electrochemical aptasensor for thrombin detection based on the host-guest recognition technique. ► Conformation-change-based electronic biosensors. ► The mono(6-O-4-aminophenoxy) heptakis(2,3-O-diacetyl) β -cyclodextrin (MPA-β-CD) was synthesized.
Highlights ► An electrogenerated chemiluminescence biosensor for Escherichia coli detection was fabricated by coating a thin layer of single wall carbon nanotubes on a graphite electrode and then covalently attached with ruthenium complex- Concanavalin A taken as the probe. ► The biosensor...
Because of the advantages of low cost, good electrical conductivity and high oxidation resistance, nitrogen-doped carbon (NDC) materials have a potential to replace noble metals in microbial fuel cells (MFCs) for wastewater treatment. In spite of a large volume of studies on NDC materials as...
Highlights ► Evaluation of the basic behaviors of biomolecules in nanospace is very important for the development of life science. ► A facile strategy for fabrication of nanoreactor was conducted by skillful confining of HRP in silica nanoshells with a reverse-micelle microemulsion method....
Highlights ► The ssDNA aptamers for Plasmodium lactate dehydrogenase (pLDH) were discovered. ► We designed the pLDH aptasensor using electrochemical impedance spectroscopy. ► Both Pv LDH and Pf LDH were selectively detected with a detection limit of 1 pM. ► The aptasensor clearly...
This article has been retracted: please see Elsevier Policy on Article Withdrawal ( http://www.elsevier.com/locate/withdrawalpolicy ). This article has been retracted at the request of the Author. This paper has been withdrawn at the request of the author as errors have subsequently been found...
Highlights ► Application of fungal peroxidase (from Coprinus cinereus ) for sulfide detection. ► Application of screen printed electrode for sulfide detection. ► Less interference of cyanide ion in enzyme inhibition compared to previous works. ► Quicker response time compared to previous...
Highlights ► The sensitivity of this Au NC-based fluorescence imaging detection method is 7–14 times higher than that of traditional staining detection methods. ► Some relatively low abundance proteins are easily detected. ► This fluorescence imaging method can distinguish between the...
Highlights ► Colorimetric solid phase tests shall be the cost-efficient approach for the democratization of multiparametric diagnosis and prognosis testing. ► We present a comparison study of four immobilization support materials for colorimetric hybridization assays in microarray format. ►...
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