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Highlights ► We developed a system with SPRI to detect individual basophils activation. ► This system detects specific activation of individual basophils induced by antigens. ► This system well allocated two spots of basophils on a sensor chip. ► SPRI has the potential as a diagnosis tool...
We report here that a hairpin-structured DNA that possesses an anti-ATP aptamer sequence successfully detected target ATP or adenosine in a temperature-dependent manner by nanoscale intramolecular displacement on the surface of a gold electrode as an extended gate of a field-effect transistor...
Highlights ► Au nanoparticle-modified TiO 2 nanotubes prepared by a one step reduction of HAuCl 4 ·3H 2 O. ► Composite material consisting of Au nanoparticle-modified TiO 2 nanotubes and hydrophobic ionic liquid facilitated electron transfer of horseradish peroxidase. ► Immersion of...
In this study, the cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to investigate the extracellular electron transfer from Escherichia coli JM109. It was demonstrated that the formal redox potential of direct electron transfer between electrode and an E. coli JM109...
Highlights ► The work describes a new compact and transportable planar patch-clamp system focusing on environmental matters, for direct on-site monitoring of the shellfish quality. ► We have combined our knowledge in planar electrophysiology and ion channels properties with microelectronics...
An optical gold nanoparticles (AuNPs)-based method was fabricated for the rapid detection of matrix metalloproteinase (MMP) activity and screening potential MMP inhibitors without sophisticated instruments. The diagnosis platform was composed of AuNPs, particular MMP substrates and...
Highlights ► CHO cells expressing wild-type EGFR and HaCaT cells showed triphasic changes of AR. ► CHO cells expressing kinase-dead EGFR showed minimum change of AR. ► Only one out of six carcinoma cell lines showed full triphasic changes of AR. ► Five out of the six cell lines showed...
Highlights ► FRET between two quantum dots causes fluorescence quenching. A binding site pre-blocking assay approach for avoiding this phenomenon is proposed. ► Pre-binding of glucose on the donor occupies the binding sites and blocks resonance energy transfer between quantum dots, protecting...
Highlights ► We report a Point of Care instrument which consists on a disposable chip. ► The device performs the identification of Salmonella spp. within 35 min. ► The entire protocol is integrated in a single chip chamber. ► A total of 29 clinical have been tested. ► The device shows...
Highlights ► We develop a rapid colorimetric droplet assays platform for double-stranded DNA. ► The incorporation of QDs and Ru(bpy) 2 (dppx) 2+ is applied in droplet for the first time. ► This platform shows great advantages in reagent consumption, detection devices and assay time.
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