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A novel and sensitive biosensor for the determination of short sequence of DNA based on flow injection (FI)-chemiluminescence (CL) system of luminol–H 2 O 2 –Cu 2+ was developed in the present work. The DNA probe labeled with copper sulfide nanoparticles (CuS NPs) could hybridize with target...
Carbon nanofibers (CNFs), with typical diameters of ∼80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and electrochemical...
Impedance measurements of cell-based sensors are a primary characterization route for detection and analysis of cellular responses to chemical and biological agents in real time. The detection sensitivity and limitation depend on sensor impedance characteristics and thus on cell patterning...
Porous nano-granule of zirconium uridine monophosphate, Zr(UMP) 2 ·H 2 O is, for the first time, synthesized under mild experimental conditions and applied to the bioconjugation of myoglobin (Mb) to realize its direct electron transfer. UV–vis and resonance Raman spectroscopies prove that Mb...
In order to protect implanted glucose sensors from biofouling, novel hydrogels (146–217% water by mass) were developed based on a copolymer of hydroxyethyl methacrylate (HEMA) and 2,3-dihydroxypropyl methacrylate (DHPMA). The porosity and mechanical properties of the hydrogels were improved...
The ability to immobilize DNA probes onto gold substrates at an optimum surface density is key in the development of a wide range of DNA biosensors. We present a method to accurately control probe DNA surface density by the simultaneous co-immobilization of thiol modified probes and...
Electrochemical label free DNA hybridization discrimination of the brain tumor sequence CK20 has been made at the gold–thiol and thiol diluent binary and ternary mixed monolayer interfaces in presence of the (Fe(CN) 6 ) 3− and double stranded DNA (dsDNA) specific cationic intercalators,...
The selection of appropriate surface materials that promote cellular adhesion and growth is an important consideration when designing a simplified neuronal network in vitro . In the past, extracellular matrix proteins such as laminin (LN) or positively charged substances such as poly- l -lysine...
Gold nanoparticles grown in situ from printed seed particles on a glass substrate have been fabricated into a biosensor array. The light-scattering properties of the resulting surfaces show sensitivity to changes in the local refractive index. Each array spot is functionalized with fibrinogen or...
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