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Cell viability assays represent an important technology in modern cell biology, drug discovery and biotechnology, where currently there is a high demand for simple, sensitive and cost-effective screening methods. We have developed a new methodology and associated tools for cell-based screening...
The immobilisation efficiency of the complexes of oligonucleotide/poly( l -lysine) on two polymeric carboxy-rich surfaces, i.e. poly(styrene/maleic acid) (PSMA) and poly(styrene/maleic anhydride) (PSMAA), has been investigated using X-ray photoelectron spectroscopy, atomic force microscopy (AFM)...
We explore the dynamic properties of multiple-beam optical traps to manipulate arrays of microstructures for biosensor applications. Multiple optical traps are generated by a virtually loss-less transformation of input phase patterns into high-intensity trapping-beams. A direct image projection...
The detection performance of conventional surface plasmon resonance (SPR) biosensors is limited to a 1 pg/mm 2 surface coverage of biomolecules, and consequently, such sensors struggle to detect the interaction of small molecules in low concentrations. The present study is attempted to propose...
Biomolecules in a confined solution environment may be subject to electrostatic forces with a range up to 100 nm, while van der Waals interaction will account for shorter-range forces. The response of two model poly(amino acids)—poly- l -lysine and poly- l -glutamic acid—has been investigated...
An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Salmonella typhimurium. A protein G layer was fabricated by binding chemically to self-assembly monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) on gold (Au) surface. The formation of...
Micro system technology offers convenient tools for the production of handling devices for small liquid volumes which can be used in cell cultivation. Here, a modular system for the rapid generation of cell suspension aliquots is presented. The system is used to produce and analyze high numbers...
An optical force measurement system for quantitating forces in the pN range between micrometer-sized objects has been developed. The system was based upon optical tweezers in combination with a sensitive position detection system and constructed around an inverted microscope. A trapped particle...
We have developed a novel optical bionanosensor platform using a supported bilayer lipid membrane (SBLM), which has generic multi-analyte sensing capabilities. The SBLM is produced using a novel combination of ordered nanostructured thin film i.e. Langmuir–Blodgett (LB) and self-assembly...
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