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Peptides derived from pro-adrenomedullin (AM) and endothelin (ET) establish two families of regulatory factors that are almost ubiquitously expressed in human tissues. Recently, some studies demonstrated that AM is expressed by all terminal cells of the lymphoid and myeloid pathways, while...
With the increased recognition that cord blood is a viable source of stem cells that can be used successfully for the treatment of blood and genetic disorders, cord blood banks are faced with the challenge of developing large inventories of diverse human leukocyte antigen (HLA) phenotypes....
CANARY cells are genetically engineered murine B cells that serve as a rapid detection system for various infectious pathogens. We are attempting to produce a stable dehydrated cellular product to make this system practical. A major source of damage to these cells during drying was identified as...
Ex vivo expanded peripheral blood progenitor cells (PBPC) have been shown to provide rapid neutrophil engraftment, and in some patients, to eliminate neutropenia after transplantation to support high-dose chemotherapy. However, the effect of expansion culture on stem cell content and potential...
Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a...
The present study was conducted with the goal of evaluating the viability of spermatozoa of the Deccan mahseer ( Tor khudree , Cyprinidae) cryopreserved using different strategies. Immotile spermatozoa pooled from 2–4 males were diluted with modified fish Ringer's solution (pH: 7.48) and...
In life sciences, an increasing number of techniques allow researchers to obtain as much data as possible from biologic specimens. One of the major problems of effective tissue preservation is to retain good tissue morphology and to preserve the nucleotide (DNA/RNA) and protein content of a...
Recent focus of the phenomenon of cryopreservation-induced delayed-onset cell death (CIDOCD) has been linked to molecular-based cellular responses. Current literature suggests that along with caspases, additional proteases such as calpains may also play integral roles in cell death. Although the...
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