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Generally, it is believed that α,β‐dehydroamino acid residues are most stable in the Z‐ form rather than in the E‐ form. In this study, it has been shown that poly‐ΔAbu exists in alternate ΔEAbu and ΔZAbu form, with &phis;, ψ values of ∼0°, 80° and 35°, 27° for the E‐ and...
Glycine‐rich proteins (GRPs) serve a variety of biological functions. Acanthoscurrin is an antimicrobial GRP isolated from hemocytes of the Brazilian spider Acanthoscurria gomesiana. Aiming to contribute to the knowledge of the secondary structure and stepwise solid‐phase synthesis of GRPs'...
Cathelicidins comprise a major family of host‐defense antimicrobial peptides in vertebrates. The C‐terminal part of the cathelicidins is bestowed with antimicrobial and lipopolysaccharide (LPS) neutralizing activities. In this work, we repot high resolution solution structures of two nontoxic...
A novel enhanced microwave‐assisted disulfide bridge formation method has been developed. To optimize the synthesis of the biologically important bicyclic peptide α‐conotoxin MII (α‐CtxMII), several cyclization methods have been tested and are discussed herein. By using m.w.‐assisted...
We recently isolated a protein disulfide isomerase (PDI) from the Rubiaceae (coffee family) plant Oldenlandia affinis (OaPDI) and demonstrated that it facilitates the production of disulfide‐knotted defense proteins called cyclotides. PDIs are major folding catalysts in the eukaryotic ER where...
Interactions of two peptides TM3 and TM4, corresponding to the third and fourth transmembrane domains of a divalent metal‐ion transporter Slc11a1, respectively, with phospholipid model membranes, including zwitterionic dimyristoylphosphatidylcholine (DMPC) and anionic...
The prediction of structural domains in novel protein sequences is becoming of practical importance. One important area of application is the development of computer‐aided techniques for identifying, at a low cost, novel protein domain targets for large‐scale functional and structural...
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