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The effect of low, medium, and high molecular weight poly(ethylene glycol) (e.g., PEG‐400, ‐6000, and ‐20,000) on the structure of the acid unfolded state of unmodified stem bromelain (SB) obtained at pH 2.0 has been studied by various spectroscopic methods. The conformation of stem...
4‐Amino‐(D3)‐trishomocubane‐4‐carboxylic acid is a constrained α‐amino acid residue that exhibits promising conformational characteristics, i.e., helical and β‐turns. As part of the development of conformational guidelines for the design of peptides and protein surrogates, the...
The structure of cytochrome c552 (Cyt‐c552) from Thermus thermophilus shows many differences to other c‐type cytochromes. The rich lysine domain close to the heme does not exist in this cytochrome, allowing us to postulate that the interaction with its redox partner must be different to the...
The vasopressin V2 receptor (V2R) belongs to the Class A G protein–coupled receptors (GPCRs). V2R is expressed in the renal collecting duct (CD), where it mediates the antidiuretic action of the neurohypophyseal hormone arginine vasopressin (CYFQNCPRG‐NH2, AVP). Desmopressin ((1‐deamino,...
The binding of manganese(III)‐tetra(4‐N‐methylpyridyl)porphyrin (MnTMpyP) with synthetic poly(dA‐dT)2, poly(dI‐dC)2, and poly(dG‐dC)2 DNAs as well as calf thymus (CT) DNA has been quantitatively studied in detail using induced CD (circular dichroism) spectroscopy in the Soret...
The ability of DNA to bind polycation yielding polyplexes is widely used in nonviral gene delivery. The aim of the present study was to evaluate the DNA compaction with a new DNA vector using Raman spectroscopy. The polyplexes result from an association of a β‐cyclodextrin polymer...
The β‐turn is a common motif in both proteins and peptides and often a recognition site in protein interactions. A β‐turn of four sequential residues reverses the direction of the peptide chain and is classified by the Φ and Ψ backbone torsional angles of residues i + 1 and i + 2. The...
The glass transition of human hair and its dependence on water content were determined by means of differential scanning calorimetry (DSC). The relationship between the data is suitably described by the Fox equation, yielding for human hair a glass transition temperature of Tg = 144°C, which is...
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