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Melting experiments were conducted on 22 DNA dumbbells as a function of solvent ionic strength from 25–115 mM Na+. The dumbbell molecules have short duplex regions comprised of 16–20 base pairs linked on both ends by T4 single‐strand loops. Only the 4–8 central base pairs of the dumbbell...
The genomic materials from some Bacillus subtilis bacteriophages are found to contain 5‐(hydroxymethyl)‐2'‐deoxyuridine in place of thymine. Phage‐encoded proteins such as transcription factor 1 specifically and preferentially bind to the minor grooves of these hmU‐containing DNA but...
The aminoacyl‐tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been...
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