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Thermal denaturation and renaturation of directly mixed and reconstituted polylysine–DNA, directly mixed polylysine–nucleohistone complexes, and NaCl‐treated nucleohistones in 2.5 × 10−4 M EDTA, pH 8.0 have been studied. At the same input ratio of polylysine to DNA, the percent of...
The helix–random‐coil transition process for poly‐γ‐benzyl‐L‐glutamate (PBG) in solvent mixtures trifluoroacetic acid/deuterochloroform (TFA/CDCl3) at different temperatures has been studied by nmr. The chemical shift behavior of the α‐CH resonances of the peptide chain and of the...
If dispersion effects are not taken into account, the dimensions of particles in suspension estimated from spectrophotometric data (slope of the log OD versus log λ plot) are too low. By measuring dispersion curves for the medium and for the suspension, a consistent estimate of the size of latex...
Certain discrepancies in measured heats of helix–coil transitions in polypeptides are examined in terms of the theory of the effect of solvent composition on transition temperatures and heats in such systems. It is found, generally, that disagreement between enthalpy measurements conducted at...
Apolar derivatives of polyethylenimine in aqueous solution have been shown previously to bind organic anions very strongly. These studies have been extended to other charged and noncharged small molecules, as well as to derivatives of polyethyl‐enimine with much reduced positive charge. From...
The chiroptical properties of L‐3‐methyl‐2,5‐diketopiperazine (L‐alanylglycyl anhydride) are examined on a theoretical model in which the electronic wave functions are obtained from semi‐empirical all‐valence‐shell molecular orbital calculations. The INDO molecular orbital model...
X‐ray diffraction and stereochemical analyses were used to study the hydrated structure of the helical amylose polymorph having a fiber repeat spacing of 8 Å. Intensity calculations using computer models confirmed six as the number of residues per turn and supported the space group P212121....
DNA from bacteriophage T7 was irradiated at long ultraviolet wavelengths in the presence of silver ions. Such treatment leads to selective production of thymine: thymine dimers in DNA. The DNA was melted and the renaturation rate was determined as a function of thymine dimer content and...
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