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A Linear peptide, GASYQDLG was synthesised and used as a model to evaluate the effects of nickel additions to increase the conformational stability. The NMR data obtained for the peptide and its histidyl derivative (H) 3 GASYQDLG(H) 3 suggest that in solution folded structures are present only...
The heat capacities (Δ C p,f ) for the temperature-induced folding of proteins: barnase, lysozyme T4, papain, trypsin, ribonuclease T1, chymotrypsin, lysozyme and ribonuclease A have been calculated from the change in solvent accessible surface area between the native state and extended...
In the present study, we investigated the interaction of the non-ionic surfactants polyoxyethylene alkyl ethers (C n E m ) with erythrocyte membranes. For this purpose we have performed hemolytic assays under isosmotic conditions with five surfactants in the 8 polyoxyethylene ether series. By...
In this work we propose that the animal–vegetal gradient spatial distribution of the IP 3 receptors observed in the Xenopus embryo can effect a uniform FGF inducting input signal, allowing for different modes of transcription of the Xbra gene, producing the differentiation of the cells of the...
The translational mobility of proteins and lipids in phospholipid bilayers is often not well described as ideal self diffusion. One of the best methods for characterizing such non-ideal diffusion is to use fluorescence pattern photobleaching recovery. In this method, the spatial gradient of the...
Several anaerobic electrochemical cells were employed to study the kinetics of iron release from pig spleen ferritin (PSF) at a bare platinum electrode. Controlled potential microcoulometry (CPM) is the principal technology used to investigate the kinetics in the absence of a mediator. A kinetic...
The conformational transition of DNA induced by the interaction between DNA and a cationic lipid vesicle, didodecyldimethylammonium bromide (DDAB), had been investigated by circular dichroism (CD) and UV spectroscopy methods. We used singular value decomposition least squares method (SVDLS) to...
We report here a quantitative analysis of the interaction of the non-ionic surfactant Triton X-100 with human erythrocyte membranes. By applying a classical treatment for the interpretation of the action of surface active compounds to the hemolytic curves, we could calculate parameters such as R...
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