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The phase transitions of dipalmitoylphosphatidylcholine (DPPC) bilayer membrane were observed by means of differential scanning calorimetry (DSC) as a function of the concentration of local anesthetics, dibucaine (DC·HCl), tetracaine (TC·HCl), lidocaine (LC·HCl) and procaine hydrochlorides...
Oxygen quenching of protein phosphorescence and activation enthalpies for the structural fluctuations underlying O 2 and acrylamide diffusion were determined for RNase T1, glyceraldehyde-3-phosphate dehydrogenase and β-lactoglobulin, which have the phosphorescing residues located in relatively...
A microscopic, reversible model to study protein crystal nucleation and growth is presented. The probability of monomer attachment to the growing crystal was assumed to be proportional to the protein volume fraction and the orientational factor representing the anisotropy of protein molecules....
We have used the enhanced green fluorescent protein (EGFP) to investigate the properties of surfactant-entrapped water pools in organic solvents (reversed micelles) with steady-state and time-resolved fluorescence methods. The surfactant used was sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and...
Any attempt to probe the membrane hydrophobic core with chemical reagents necessitates the use of reactive intermediates like carbenes and nitrenes, which can insert into CH bonds. Several photoactivable reagents based on carbenes and nitrenes have been reported. However, the high reactivity...
We have investigated a point mutant of the HIV-1 fusion peptide in a compressed monolayer at the air–water interface. A variety of surface sensitive techniques were applied to study structural features under conditions mimicking the hydrophobic/ hydrophilic environment of a biomembrane....
Analysis of protein–protein interactions in highly concentrated solutions requires a consideration of the non-ideality in such solutions which is expressed by the virial coefficients. Different equations are presented to estimate effects of the thermodynamic non-ideality on the macromolecular...
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