New! You can now keep track of new articles from Biological Chemistry on your personalized homepage!
Abstract Small interfering RNAs (siRNAs) represent RNA duplexes of 21 nucleotides in length that inhibit gene expression. We have used the human gene-external 7S K RNA promoter for synthesis of short hairpin RNAs (shRNAs) which efficiently target human lamin mRNA via RNA interference (RNAi). Here...
Abstract The kinetic characterization of horseradish peroxidase (HRPC) substrates is difficult because the reaction products are free radicals. The application of a spectrophotometrical method, which is based on determining the time necessary for a given quantity of L-ascorbic acid to be consumed...
Abstract MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell...