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Abstract A clone was isolated from a cDNA library from early embryos of Xenopus laevis that codes for a highly charged protein containing 339 amino acids. Two putative nuclear localization signals could be identified in its sequence, but no other known motifs or domains. Closely related ORFs are...
Abstract Methods to assess metabolism are important analytical tools in neuroscience. The fluorophore nicotinamide adenine dinucleotide (NADH) is a parameter of cellular metabolism. NADH fluorescence was measured using a laserbased fluorescence detector with spectral and temporal filters....
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Abstract Studies of oxidative stress have classically been performed by analyzing specific, single antioxidants. In this study, susceptibility to oxidative stress in the human keratinocyte cell line NCTC2544 exposed to hydrogen peroxide (H2 O2) was measured by the TOSC (total oxyradical...
Abstract Epidemiological studies suggest that βcarotene is able to modulate the risk of cancer. A number of in vitro studies reported that βcarotene inhibits the growth of cancer cells; however, so far little is known about the molecular mechanisms of the antiproliferative effect of βcarotene....
Abstract Heterodimeric calpain, consisting of the large (80 kDa) and the small (30 kDa) subunit, was isolated and purified from human erythrocytes by a highly reproducible fourstep purification procedure. Obtained material is more than 95% pure and has a specific activity of 6 7 mU/mg. Presence...
Abstract The thiol/disulfide oxidoreductases play important roles in ensuring the correct formation of disulfide bonds, of which the DsbE protein, also called CcmG, is the one implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. The soluble,...
Abstract A new control of mitochondrial membrane potential ΔΨm and formation of reactive oxygen species (ROS) is presented, based on allosteric ATPinhibition of cytochrome c oxidase at high intramitochondrial ATP/ADP ratios. Since the rate of ATP synthesis by the ATP synthase is already maximal...
Abstract In contrast to signal generation and transmission, the mechanisms and molecules that negatively regulate receptor tyrosine kinase (RTK) signaling are poorly understood. Here we characterize Mig-6 as a novel negative feedback regulator of the epidermal growth factor receptor (EGFR) and...
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