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Enzymatic acylation of rutin and esculin with aromatic, aliphatic and aryl-aliphatic acids using Candida antarctica lipase in tert amyl alcohol as solvent was investigated under low water content. Whatever the acyl donor used, the conversion yields and initial rates for esculin were higher than...
Alpha-Chymotrypsin was found to show a 119% increase in activity after three phase partitioning. The k cat /K m of the partitioned enzyme ( TPP-C ) for hydrolysis of Bz-Tyr-OEt in aqueous medium at 25°C was found to be 48.3×10 4 mM −1 min −1 as compared to the corresponding value of...
1,4-Dihydropyridines possess a broad range of biological activities, such as the ability to control the influx of calcium into cells, as well as neuroprotective, antineurodegenerative, cognition and memory enhancing, anti-inflammatory, antiviral and many other properties. Chirality plays an...
Mono- and dilauroyl arabitols, ribitols, xylitols and sorbitols were synthesized batchwise or continuously at 50°C or 60°C by condensation catalyzed by an immobilized Candida antarctica lipase in acetone. Continuous production was realized using a system where a column packed with sugar alcohol...
The ability of dehydrated baker's yeast (Sigma, type II) to carry out oxidation reactions was investigated using a mixture of ( S )- and ( R )-enantiomers of 2-heptanol operated in a biphasic system with hexadecane as the organic layer. The commercial material could be used without preliminary...
Biochemical properties of inulosucrase from Leuconostoc citreum CW28, a potential biocatalyst for inulin synthesis, were determined in order to select optimal reaction conditions. The hydrolysis reaction was about 3.5 times more efficient than the transferase reaction. It was found that high...
We have recently found that toluene para -monooxygenase (TpMO) of Ralstonia pickettii PKO1 (encoded by tbuA1UBVA2C ) performs successive hydroxylations of benzene ( Appl. Environ. Microbiol. 70: 3814, 2004) as well as hydroxylates toluene to a mixture of 90% p -cresol and 10% m -cresol which are...
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