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The review spans a decade of research work ranging from 1989 to 1999, and reviews numerous articles dealing with lipase purification. Different purification techniques and their relation to the source of the enzyme are examined. It has been observed that some proteins tend to be unstable in...
Cells of Rhodococcus erythropolis DCL14 present a limonene epoxide hydrolase activity when grown on terpenes, which enables them to convert cis -limonene-1,2-epoxide to limonene-1, 2-diol. Trans -limonene-1,2-epoxide is only converted when no cis is present. An organic/aqueous system was...
Recent studies by Reetz et al. (Reetz, M.T., Zonta, A. and Simpelkamp, J. (1996a) Biotechnology and Bioengineering , 49 , 527-534) have shown that the catalytic activity of lipase encapsulated in sol-gel materials, in esterification reactions, depends on many parameters such as the presence of...
A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems....
Alkaline p -nitrophenylphosphate phosphatase ( p NPPase) from the halophilic archaeobacterium Halobacterium salinarum (earlier halobium ) was solubilised in reversed micelles of hexadecyltrimethylammoniumbromide (CTAB) in cyclohexane, with 1-butanol as co-surfactant. The hydrolysis of p...
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