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Cross-linked enzyme crystals (CLECs®) are a novel form of immobilised biocatalyst designed for application in large-scale biotransformation processes. In this work we review the production and characterisation of CLECs® prepared from three enzymes (yeast alcohol dehydrogenase I (YADHI), Candida...
Chemico-enzymatic methods are a promising approach to obtain various biologically active compounds, including enantiomerically pure substances. Entrapment in gels is one of the most convenient methods to stabilize enzymes for their application in water/organic media. In this study, proteases...
Several hydrolases have been employed for the resolution, carried out both in aqueous and organic media, of compounds of pharmaceutical interest. Among these are the mucolytic drug trans -sobrerol, the precursors of ( R )- and ( S )-broxaterol (a β-adrenergic stimulant), of ( R )- and ( S...
Biotransformations are now accepted as a methodology for the preparation of fine chemicals. The majority of enzyme-catalysed reactions involve the use of hydrolases, especially lipases, in hydrolysis or transesterification reactions. Over the last few years, other interesting processes have...
Biocatalytic production of several key chiral intermediates in the synthesis of anti-inflammatory, anti-viral and anti-leukaemic agents is described. These include (i) a nucleoside oxidase from Stenotrophomonas maltophilia to oxidise unnatural purine nucleosides, (ii) a serine-type protease from...
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