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A rapid and sensitive assay for the detection of lipase activity is described. The method is based upon the increase in absorbance at 360 nm due to the formation of the 2,4-dinitrophenolate anion during the enzymatic hydrolysis of 2,4-dinitrophenyl butyrate. The substrate is used in an emulsified...
The kinetics of lipid-hydrolysis by Candida rugosa lipase was investigated in a membrane reactor and in an emulsion system. Two models were chosen to describe the kinetics of the enzyme: (1) The hydrolysis of triglycerides to fatty acids was considered to be a chain reaction with the intermediary...
An aqueous two-phase system of dextran and polyethylene glycol was investigated as a reaction medium for pig liver microsomes in order to find out if the partition of the microsomes, of the substrate 7-ethoxycoumarin and of the product 7-hydroxycoumarin favoured any biotechnological perspectives....
The synthesis of optically active ethyl 4-chloro-3-X-butanoate derivatives la-d (X = OH, a; OCOCH 3 , b; OCOC 3 H 7 , c; OCH 2 C 6 H 5 , d) was realized using various biocatalytic approaches such as microbiological reduction of ethyl 4-chloro-3-oxobutanoate 2 with lactic acid bacteria, hydrolysis...