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Young adult and weanling pigs were challenged with the New York 99 strain of West Nile virus through the bite of infected mosquitoes. Each of six adult pigs seroconverted, but virus was isolated from serum of only one pig following challenge. Three of five weanling pigs developed viremia, with...
The movement protein (MP) of Brome mosaic virus (BMV) depends on the coat protein (CP) to mediate the cell-to-cell movement of BMV and CCMV(B3a), a recombinant Cowpea chlorotic mottle virus (CCMV) expressing BMV MP. Previous studies identified gain-of-function mutations in the central region of...
Two envelope glycoprotein (E rns and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-PCR and sequenced directly from 158 specimens collected between 1989 and 2003 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the E rns region...
Hepatitis C virus (HCV) genome contains a 3′noncoding region (3′NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3′NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of...
Tissue-cultured seedlings of calla lily ( Zantedeschia spp.) with mild mosaic symptom were observed in Taiwan. A new potyvirus, Zantedeschia mild mosaic virus (ZaMMV), was identified in the diseased plants based on the result of ELISA, virion morphology and sequence data. In the host range test,...
It has been suggested that Citrus viroid IV (CVd-IV) be classified as a species within the genus Cocadviroid . This relationship was based on the presence of a terminal conserved hairpin (TCH) and absence of a terminal conserved region (TCR) as specific structural motifs in common with isolates...
A minimal bovine leukemia virus (BLV) RNA packaging sequence (E) required for heterologous RNAs to be packaged into BLV particles was analyzed. The BLV E was inserted into a non-viral vector, pLacZ, in order to determine if packaging of the non-viral vector RNA would occur. The construct was...
A novel reverse transcription-multiplex polymerase chain reaction (RT-multiplex PCR) assay that can detect enteroviruses, hepatitis A and E viruses and influenza A virus from various hosts (avian species, human, swine and horse) was developed. The identification of that group of viruses was...
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