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At present, 13C-MFA is a primary method for quantitatively characterizing intracellular carbon fluxes in cells in vivo under steady-state conditions. The method has been successfully used to investigate both the fundamental characteristics of prokaryotic and eukaryotic cell metabolism and to...
Electrobiosynthesis conducted by microorganisms represents a new technology with great potential. This review considers mechanisms of direct electron transfer from cathode to bacterial cell and a number of anaerobic processes catalyzed with such transport: the biosynthesis of hydrogen, methane,...
The work reports the construction of Escherichia coli strain MG1655 derivatives with deleted genes that encode fumarases (fumAC, fumB, and fumABC) via the phage lambda-mediated recombination system. It has been demonstrated that the deletion of fumB gene had almost no effect on strain growth...
The activity of chorismate synthase, the terminal enzyme of the common aromatic pathway, is absolutely dependent on reduced flavin mononucleotide. The bifunctional chorismate synthase of Saccharomyces cerevisiae (product of the ARO2 gene) can reduce flavin in a reaction that involves NADPH, in...
Transposons are mobile genetic elements that are part of the genomic DNA of numerous organisms and belong to two classes. Unlike class I transposons, class II DNA transposons do not use the stage of RNA synthesis in their transition; they perform it by the cut-and-paste mechanism or with a...
The capacity of a recombinant Yarrowia lipolytica yeast strain VKPM Y3753 for succinic acid biosynthesis in a laboratory bioreactor at low pH has been studied. The batch and repeated-batch modes of fermentation of the strain were compared. The optimal conditions for repeated-batch fermentation...
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