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Based on the technology for producting virus-like particles of a killer strain of Saccharomyces cerevisiae Y-448 yeast, which includes stages of extraction, concentration, and chromatographic purification, experimental preparation samples were obtained and their properties were researched. The...
A review of modern works on the creation of biotechnological processes using cells immobilized on different carriers is presented. General material requirements are given for for immobilization mainly performed by absorption and mechanical fixation methods. The results of studies on cell...
A combination of cultural medium and nourishment providing a high yield of target glycated forms of recombinant darbepoetin alfa produced by CHO cell culture was selected. The influence of different substances—precursors of glycans in glycosylation reactions—on the yield of highly sialylated...
The recombinant E7 oncoprotein gene of type 16 human papilloma virus (conjugated with the heat shock protein 70 (E7(16)-HSP70)) was expressed in Saccharomyces cerevisiae yeast cells. A number of chromatographic isolations were conducted to obtain the target protein and its primary identification...
A new immunochromatographic assay was developed for serodiagnosis of tuberculosis based on the interaction of specific anti-Mycobacterium tuberculosis immunoglobulins that are present in the blood serum with a label-antigen conjugate followed by the binding of the resulting complex to the...
In this review, existing succinic acid manufacturing methods are reviewed. The economic status and the prospects for chemical and microbiological means of biosuccinic acid production are also presented. Possible approaches to lower production costs via improvements in known technologies, as well...
The Mycobacterium tuberculosis Rv1248c (kgd) gene has been expressed in the recombinant Escherichia coli strain with the inactivated pathways of mixed-acid fermentation and anaerobic generation of acetyl-CoA, and also with modified system of glucose transport and phosphorylation, and altered...
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