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The fermentation of a Saccharomyces cerevisiae strain producing recombinant spidroin IF9 was optimized. A simplified two-stage scheme of the process was developed; the effect of sucrose, glucose, fructose, and galactose on the efficiency of the process was investigated. The optimal concentration...
The recombinant human interferon (IFN) beta-1a was modified with polyethylene glycol (PEG). The reaction was performed with the use of activated linear butyraldehyde PEG derivative with a molecular weight of 30 kDa. As a result of the multifactorial experiment, the correlation between the...
In this review, the metabolic engineering approaches including those used by the authors in creating phenylalanine producers based on Escherichia coli were systematized. Optimization of the amino acid biosynthesis was conducted in order to obtain significant quantities of phenylalanine and to...
Expression of the Saccharomyces cerevisiae proteasomal genes is regulated coordinately by ScRpn4 transcription factor and its binding site known as PACE. Genomic analysis demonstrates that only Saccharomycetes yeasts carry ScRpn4-orthologous genes and PACE-like elements in the promoters of...
Cloning of aspartase gene (L-aspartate ammonia-lyase) from the natural isolate Escherichia coli VKPM V-7188 in the pLATE31 plasmid composition and its expression under the control of the T7 phage promoter in Escherichia coli BLR (DE3) cells was performed. It was shown that the aspartase content...
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