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This review analyzes the methodology available for the creation and analysis of metagenomic libraries, targeted search for genes encoding certain types of enzymes, and also phenotypic selection of clones containing functioning genes with consequent identification by sequencing, cloning, and...
A DNA fragment containing a promoter-operator and structural parts of the uridine phosphorylase gene from Shewanella oneidensis MR-1 was cloned. Cross-heterological expression of the udp genes from Sh. oneidensis MR-1 and Escherichia coli under the control of authentic regulatory regions is...
Approaches to the molecular design of antiviral agents based on the principals of adequacy and mimicry to biopolymers have been discussed. The approaches permit for the reproduction of pharmaceutically valuable properties of the polymeric basis (core) and to create novel nongenetic “programs” of...
For the first time, a diagnostic test system for the determination of drugs, in particular, cannabinoids, in human urine was constructed on the basis of the latex agglutination reaction. For this purpose, polystyrene microspheres with a diameter of 0.6 μm and a narrow particle size distribution...
Yalf tomato plants have been transformed with a gene for thaumatin II from Thaumatococcus daniellii Benth. The nucleotide sequence for thaumatin II cDNA was cloned in the pBI121 vector under the control of the CaMV 35S promoter of cauliflower mosaic virus. Expression of the thaumatin II gene was...
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