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The effect of exogenous recombinant interleukin 10 on the replication of low-passage HIV-1 strains in blood-derived monocytes and monocyte-derived-macrophages (MDMs) was examined at various stages of cell maturation after adherence to the plastic substrate. Interleukin 10 inhibited extracellular...
A cohort of 39 vertically infected children (class N, A, B, and C of the CDC HIV classification for pediatrie infection) was studied by virus isolation and non-syncytium inducing (NSI)/syncytium inducing (SI) HIV-1 phenotype evaluation. The HIV-1 isolates were recovered from PBMCs and the MT-2...
This article demonstrates that human immunodeficiency virus type 1 (HIV-1) gp120 amplifies the activity of tumor necrosis factor Î±(TNF-Î±), a cytokine that stimulates HIV-1 replication through activation of NF-kB. In CD4-positive Jurkat cells, gp120 potentiates TNF-induced NF-kB activation....
Productive infection by the LAV strain has been demonstrated in T cell precursors at different stages of intrathymic development, while viral replication in thymic epithelial cells is still controversial. In this article we show that epithelial cell cultures derived from the medullary component...
The mechanism of inhibition of HIV Ba-L replication by interleukin 10 (IL-10) in primary monocytes and macrophages at various stages of maturation was investigated using semiquantitative PCR for reverse-transcribed HIV DNA, and Northern hybridization for HIV mRNA expression in comparison with...
Correlates of progression of human immunodeficiency virus (HIV) infection to AIDS include the reduction in CI4 + T cells and the emergence of syncytium-inducing (SI) HIV variants. It has been suggested that progressive defects in interleukin 2 (IL-2), IL-12, and IFN-Î³ production (type 1...
We used semiquantitative RT-PCR to monitor the expression of mRNA encoding cytokines (IL-1Î²6,IL-6,TNF-Î±, and IL-10) and IFN-Î³ in fresh isolated peripheral blood mononuclear cells (PBMCs), lymph node mononuclear cells (LNMCs), and mononuclear cells obtained after bronchoalveolar lavages...
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