RSC Advances

Li, Fang; Lu, De-Yun; Zhong, Qiu; Tan, Fang; Li, Wenfeng; Liao, Wei; Zhao, Xin

doi: 10.1039/c9ra08789cpmid: 35492553

This study was conducted to investigate the preventative effect of Lactobacillus fermentum HFY06 on carbon tetrachloride (CCl4)-induced liver injury in Kunming mice. Mice were treated with HFY06, then liver damage was induced using CCl4. Evaluation indicators included the activities of aspartate aminotransferase (AST), triglycerides (TG), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in serum; cytokines levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in serum; and related gene expressions of nuclear factor-κB (NF-κB), TNF-α, cyclooxygenase-2 (COX-2), copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), and catalase (CAT). Liver tissue was stained with hematoxylin and eosin for pathological analysis. Compared with the model group, HFY06 reduced the liver index, increased the serum SOD and GSH-Px activities, and reduced the AST, TG, and MDA activities in the mice. Inflammation-related IL-6, TNF-α and IFN-γ levels were also reduced after treatment with a high dose of HFY06. Pathological observation showed that CCl4 damaged the mouse livers, which were significantly improved after treatment with silymarin and HFY06. qPCR also confirmed that the high dose of HFY06 (109 colony-forming units [CFU] per kg per day) upregulated the mRNA expression of the antioxidant genes, Cu/Zn-SOD, Mn-SOD, and CAT, in the liver tissue and downregulated the mRNA expression of the inflammatory factors, NF-κB, TNF-α and COX-2, but HFY06 was less effective than silymarin. These findings indicate that HFY06 prevented CCl4-induced liver damage in vivo but was less effective than silymarin. Thus, HFY06 may have a potential role in treating liver diseases.

Zhang, Yanan; Jin, Yiliang; Cui, Haixia; Yan, Xiyun; Fan, Kelong

doi: 10.1039/c9ra09021epmid: 35492517

Nanozymes, a type of nanomaterial with intrinsic enzyme-like activities, have emerged as a promising tool for disease theranostics. As a type of artificial enzyme mimic, nanozymes can overcome the shortcomings of natural enzymes, including high cost, low stability, and difficulty in storage when they are used in disease diagnosis. Moreover, the multi-enzymatic activity of nanozymes can regulate the level of reactive oxygen species (ROS) in various cells. For example, superoxide dismutase (SOD) and catalase (CAT) activity can be used to scavenge ROS, and peroxidase (POD) and oxidase (OXD) activity can be used to generate ROS. In this review, we summarize recent progress on the strategies and applications of nanozyme-based disease theranostics. In addition, we address the opportunities and challenges of nanozyme-based catalytic theranostics in the near future.

Li, Yongqian; Mao, Yunlong; Xiao, Chi; Xu, Xiaoli; Li, Xueyong

doi: 10.1039/c9ra09188bpmid: 35492551

pH is a critical parameter used to specify the acidity or alkalinity of an aqueous solution in chemistry, food processing, and medical care. In this study, a conductimetric-type micro pH sensor has been achieved using PANI membrane fabricated on a flexible substrate film aiming to monitor wound healing. The sensor is based on the incorporation of a polyaniline (PANI) membrane, interdigital electrode, and polyimide (PI) substrate. PANI was doped with dodecyl benzene sulfonic acid (DBSA) to obtain good conductivity. The electrodes were patterned on the PI film by etching. The contact area between the PANI and interdigital electrodes improves the responsiveness of the pH sensor. A sensitivity of 58.57 mV per pH over the entire pH range from 5.45 to 8.62 was obtained experimentally, along with a superior repeatability of 8% FS (full scale) and a temperature drift of 6.8% FS. This micro flexible pH sensor aims to monitor the pH value of wound healing, which also facilitates the realization of online monitoring of the pH for telemedicine, food safety, and home health care.

Qi, Xianjin; Li, Yongkui; Wei, Longhua; Hao, Fengyan; Zhu, Xing; Wei, Yonggang; Li, Kongzhai; Wang, Hua

doi: 10.1039/c9ra06568gpmid: 35492560

The typical disposal of high-arsenic waste acid is at the expense of discharging a large quantity of hazardous solid waste, resulting in secondary pollution of arsenic. We propose a modified lime/ferric salt method for high-arsenic waste acid disposal by the stepwise formation of gypsum and scorodite at atmospheric pressure. The sulfuric acid in the high-arsenic waste acid is first removed by calcium carbonate generating gypsum, and then the arsenic in the solution is precipitated in form of scorodite. Gypsum with an arsenic leaching concentration below 5 mg L−1 is obtained at a final pH of 0.5 in the calcium carbonate neutralization stage. In the second stage, the optimal conditions including a starting pH of 2.0, an Fe/As ratio of 1.5, a reaction temperature in the range of 80–90 °C and a reaction time equal to or longer than 8 hours provide an arsenic removal efficiency of 95.34% by the formation of well-crystallized and environmentally stable scorodite with grain sizes in a range of 1–5 μm. The proposed process offers a promising and facile solution for the low-cost disposal of high-arsenic waste acid in the nonferrous metallurgical industry, which enables an efficient arsenic removal with the good accessibility of chemical reagents and facilities.

He, Qianqian; Xie, Chunsheng; Gan, Dexin; Xiao, Chun

doi: 10.1039/c9ra09253fpmid: 35492530

In recent years, persulfate (PS) has been widely studied as a promising oxidant. In this work, a new K-Fe2O3 catalyst was synthesized via a facile impregnation method. K-Fe2O3 samples were utilized as heterogeneous photocatalysts for the degradation of aquatic organic pollutants (rhodamine, RhB, and ciprofloxacin, CIP). The catalysts showed excellent catalytic activity in the presence of PS under the irradiation of visible light, owing to the generation of SO4˙− and ·OH active radicals. The degradation ratio and COD removal ratio for RhB were 99.8% and 88.3%. More importantly, the system retained a high degradation activity for RhB within a wide operating pH range of 2.9–10. The results of cycling degradation experiments confirmed that the K-Fe2O3 catalyst was stable and recoverable. Large-scale experiments for treating dye wastewater under irradiation by natural sunlight were carried out, showing that this study can provide a new perspective for the treatment of wastewater.

Steinebrunner, David; Schnurpfeil, Günter; Wöhrle, Dieter; Wittstock, Arne

doi: 10.1039/c9ra08841epmid: 35492516

A series of singlet oxygen sensitizing hybrid materials is reported consisting of a zinc(ii) phthalocyanine (ZnPc) derivative immobilized on nanoporous gold leafs (npAu) with various pore sizes. The resulting photocatalytic coatings exhibit a thickness of around 100 nm and pore sizes between 9–50 nm. Herein, we report the synthesis and characterization of those hybrid materials which were synthesized by functionalization of npAu leafs by an azide terminated alkanethiol self-assembled monolayer (SAM) and subsequent copper catalyzed azide–alkyne cycloaddition (CuAAC). The characterization of the samples morphology included scanning electron microscopy (SEM), UV-Vis spectroscopy as well as energy dispersive X-ray spectroscopy (EDX). The morphology–reactivity relationship was investigated employing the hybrid photocatalysts in the photooxidation of diphenylisobenzofuran (DPBF) as selective singlet oxygen quencher. An increasing photocatalytic activity was found for smaller pore sizes up to 15 nm, due to the gain in specific surface area concomitant with an increasing amount of immobilized photosensitizer, completely dominating the effect of the higher spectral overlap caused by the shift of the plasmon resonance of npAu, until mass transport and diffusion limitation gets predominant for pore sizes below 15 nm.

Naga, Naofumi; Fujioka, Shun; Inose, Daisuke; Ahmed, Kumkum; Nageh, Hassan; Nakano, Tamaki

doi: 10.1039/c9ra09684apmid: 35492514

Porous polymers have been synthesized by Michael addition reactions of multi-functional acrylate and diamine or dithiol compounds. Aza-Michael addition reaction of multi-functional acrylate, trimethylolpropane propoxylate triacrylate (TPT) and hexamethylene diamine (HDA) in dimethyl sulfoxide (DMSO) successfully yielded the porous polymer. The porous structure was characterized by connected globules or co-continuous structure, and could be controlled by the reaction conditions. Mechanical properties of the porous polymers were investigated by compression test. The porous polymers with co-continuous structure showed higher Young's modulus than those with connected globules. The porous polymer absorbed some organic solvents, especially CHCl3. The porous polymer as prepared in DMSO state showed coloring induced by Christiansen filter effect depending on the reaction time and observation temperature. The thio-Michael addition reaction of TPT and 1,6-hexanedithiol (HDT) in DMSO using different base catalysts also yielded the porous polymer. The porous structure could be controlled by the catalysts amount when the reaction was initiated by a photo-base generator as the base catalyst. The present reaction systems make it possible to synthesize the porous polymers with simple process without phase separator.

Takano, Kaori; Nyu, Takashi; Maekawa, Tatsuhiro; Seki, Takashi; Nakatani, Ryuichi; Komamura, Takahiro; Hayakawa, Teruaki; Hayashi, Tomohiro

doi: 10.1039/c9ra09043fpmid: 35492547

An instrumentation technique for real-time, in situ and real space observation of microphase separation was proposed for ultra-high molecular weight block copolymer thin films (1010 kg mol−1, domain spacing of 180 nm) under high solvent vapor swelling conditions. This is made possible by a combination of a homebuilt chamber which is capable of supplying sufficient amount of vapor, and force–distance curve measurements which gives real-time swollen film thickness and allow active feedback for controlling the degree of swelling. We succeeded in monitoring the domain coarsening of perpendicular lamellar structures in polystyrene-block-poly(methyl methacrylate) thin films for eight hours via tapping mode imaging. During the annealing process, the thickness reached a maximum of 8.5 times that of the original film. The series of temporal real space topographic images obtained via this method allowed us to study, for the first time, the growth exponent of the correlation length under solvent vapor annealing.

Serag, Ahmed; Baky, Mostafa H.; Döll, Stefanie; Farag, Mohamed A.

doi: 10.1039/c9ra07841jpmid: 35492526

Herbs of the Umbelliferae family are popular spices valued worldwide for their many nutritional and health benefits. Herein, five chief umbelliferous fruits viz., cumin, fennel, anise, coriander and caraway were assessed for its secondary metabolites diversity along with compositional changes incurring upon roasting as analyzed via ultra-high performance liquid chromatography coupled to photodiode array and electrospray ionization mass detectors UHPLC-qToF/MS. A total of 186 metabolites were annotated, according to metabolomics society guidelines, belonging mainly to flavonoids, fatty acids and phenolic acids. Multivariate models viz., PCA, HCA and OPLS-DA were further employed to assess fruits' heterogeneity in an untargeted manner and determine mechanistic changes in bioactive makeup post roasting viz., glycosidic cleavage, lipid degradation and Maillard reaction. Finally, the fruits' antioxidant activity showed decline upon roasting and in correlation with its total phenolic content. This study presents the first complete map of umbelliferous fruit metabolome, compositional differences and its roasting effect.

Nishiyabu, Ryuhei; Takahashi, Yuki; Yabuki, Taro; Gommori, Shoji; Yamamoto, Yuki; Kitagishi, Hiroaki; Kubo, Yuji

doi: 10.1039/c9ra08208epmid: 35492531

The self-assembly of polyvinyl alcohol (PVA) and benzene-1,4-diboronic acid (DBA) is employed as a sol–gel method for one-step fabrication of hydrogel coatings with versatile functionalities. A mixture of PVA and DBA in aqueous ethanol is prepared as a coating agent. The long pot life of the mixture allows for the coating of a wide range of materials with hydrogel films by simple cast- and dip-coating techniques. The resultant films show negligible dissolution in water and the intrinsic hydrophilicity of PVA provides the films with functional properties, such as improved antifogging property and resistance to protein and cell fouling. The self-assembling process shows adaptive inclusion properties toward nanoscale materials, such as metal–organic coordination polymers and inorganic nanoparticles, affording composite films. Furthermore, the coating film exhibits a unique secondary functionalization reactivity toward boronic acid-appended fluorescent dyes, through which a variety of materials are converted into fluorescent materials.