Pathology International

Mukai, Makio; Torikata, Chikao; Ibi, Hisami; Hata, Junichi; Naito, Makoto; Shimoda, Tadakazu; Kageyama, Keizo

doi: 10.1111/j.1440-1827.1986.tb02225.xpmid: N/A

Three cases of infantile digital fibromatosis were studied by electron microscopy and immunohistochemistry. The tumor was made up equally of myofibroblasts containing long narrow bundles of microfilaments with dense bodies. Another striking feature was the intracytoplasmic electron‐dense inclusion. The inclusions were constituted of packed fibrils similar to those of the long bundles. At the periphery of these inclusions were seen prominent felt‐like filamentous structures; the diameter varied from 5–12nm. The 10 nm filaments were seen most abundantly in the cytoplasm surrounding the inclusions, but sometimes they were also found within the inclusions themselves, mainly at their periphery. These findings suggest that the inclusions may represent an abnormal accumulation of cytoskeletal proteins. On the other hand, various kinds of Immunohistochemical study showed that actin, actomyosin, myosin, and vimentin were distinctly observed in cytoplasms of tumor cells. They were located fringing the intracytoplasmic inclusions, but the inclusion themselves showed negative findings, thus indicating a hollow‐like staining pattern. These data can be synthesized as follows. The inclusion results from an accumulation of cytoskeletal proteins (actin‐myosin complex and vimentin, at least), and antigeniclty seems to be lost by for a certain mechanism or in the degenerative process.

Nonomura, Akitaka; Ohta, Goroku

doi: 10.1111/j.1440-1827.1986.tb02226.xpmid: 3811907

Eighty five cases of malignant lymphoma were surveyed for the presence of angioinfiltrative and angiodestructive growth pattern of lymphold cells with areas of necrosis and granulomatous appearance simulating lymphomatoid granulomatosis (LYG). LYG‐like changes were observed in 13 of 85 cases (15%). The most frequent histology was diffuse large cell lymphoma which occurred in 8 cases (4 non‐cleaved, 3 cleaved, and one immunoblastic), followed by three diffuse pleomorphic lymphoma, one diffuse medium‐sized lymphoma, and one Hodgkin's disease of mixed cellularity. The most frequent involving site was the lung, as well as the soft tissue. Composition of lymphoid infiltrate varied markedly from case to case and from area to area in the same case. One was solely composed of monotonous atypical cells, other pleomorphic cells including benign lymphocytes, plasma cells, leukocytes, and histiocytes with a varied number of atypical reticuloendothelial cells. The latter histologic appearance was just similar to LYG. Veins were more frequently affected than arteries. The present study indicates that, although the angioinflltrative and angiodestructive growth pattern of atypical lymphoreticular cells with granulomatous appearance is considered necessary for the diagnosis of LYG, it often occurs in malignant lymphoma. Therefore, one should consider the possibility of malignant lymphoma before making the diagnosis of LYG, especially in the specimens taken from the lung and soft tissue.

Takaki, Keizo

doi: 10.1111/j.1440-1827.1986.tb02227.xpmid: 3811908

Histopathological analysis of reactive lymphoid hyperplasia (RLH) and malignant lymphoma (ML) of the stomach was made based on the observations of lymphoid follicles appearing in gastric mucosa. The materials used for study consisted of 28 stomachs without RLH or tumor obtained from either operation or autopsy, 18 stomachs with RLH, and 46 stomachs with ML. Lymphoid follicles appeared soon after birth, increased with mucosal atrophy, and decreased in mucosa with intestinal metaplasia. They possessed a sinusoidal structure and appeared in the muscularis mucosae intimately related to the lymphatic flow. Excepting for the significant proliferative nature, the structural evolution of lymphoid follicles in RLH was similar to that in atrophic mucosa. They showed, however, a wide spectrum of changes containing cases with simple reactive changes to those with prelymphomatous changes. As to ML, the large cell type demonstrated a destructive proliferation, while both small and medium‐sized cell types showed proliferation and infiltration preserving their original structure. In the superficial type, ML was frequently accompanied by RLH and mucosal atrophy. The histological features of ML consisted of not only the characteristics of tumor cells but also the proper mucosal changes including lymphoid follicles.

Nakano, Masayuki

doi: 10.1111/j.1440-1827.1986.tb02228.xpmid: 3811909

Examination of hepatic fibrosis Induced by choline deficient diet showed the histogenesis of early flbrosis and septa formation. Male rats were fed a choline deficient diet. Groups of three were sacrificed after 1, 2, 3, 6, 8, 12, and 13 weeks. Control groups of three rats were sacrificed after 6, 8, and 12 weeks. All choline deficient rats showed initial steatosis around the portal tracts. The fat droplets were small at first and gave a microvesicular appearance. As they grew, they gathered around the terminal hepatic venule by the 3rd week. Then, retlcular fibers developed around the hepatocytes in the centrolobular area and fibroses connected the terminal hepatic venules. The fat droplets became larger and made nodular masses around the portal tract. These compressed the fibroses to the periphery where they formed septa. After 12 weeks, regenerating nodules developed. Electron microscopy showed that fat storing cells helped cause pericellular fibrosis.

Yonezawa, Suguru; Shibata, Masao; Shimizu, Takeshi; Nakamura, Takao; Sato, Eiichi

doi: 10.1111/j.1440-1827.1986.tb02229.xpmid: N/A

An isolectin from Griffonia simplicifolia (GS) seed—GSI‐A4—stained the outer aspect of glomerular tuft (GT) intensely in the kidneys of ICR, C57BL/6J, BALB/c and NSY mice. Loss of the GSI‐A4 staining was observed in the sclerotic areas of glomeruli in diabetic mice (NSY mice). This interesting staining will be useful for the analysis of the constitutions of GT in various experimental models of renal glomerular diseases in mice.

Matsuo, Kenji; Tsuchiyama, Hideo

doi: 10.1111/j.1440-1827.1986.tb02230.xpmid: 3028037

Four adrenocortical adenomata with primary aldosteronism (Conn's adenomata) were examined by light and electron microscopy employing cell culture methods. Unstimulated cells existed as a unit of the clusters, but Conn's ademona cells reacted to ACTH. The cultured cells produced mostly cortisol under ACTH‐stimulation as time passed. Lipid droplets in their cytoplasm decreased in number and the smooth endoplasmic reticulum (sER) was well‐developed with dilated, anastomosing tubule. Moreover, mitochondria sometimes had tubulovesicular cristae. It is assumed that Conn's adenoma cells are hybrid type cells, which are intrinsically capable of producing cortisol as well as aldosterone.

Nonomura, Akitaka; Saito, Katsuhiko; Kono, Naoko; Ohta, Goroku

doi: 10.1111/j.1440-1827.1986.tb02231.xpmid: 3811910

Two cases with Mallory body (MB)‐like cytoplasmic hyalins in cells, which were quite uncommon as MB‐having cells, were presented. The cases were 61‐and 59‐year‐old males. MB‐like hyalins were found in large cell carcinoma cells of giant cell type of the lung. The cells containing MB‐like hyalins frequently showed a hydropic swelling of the cytoplasm with pyknotic, or wrinkled nuclei. Histochemical properties of the hyalins were just similar to those of hepatic MBs in alcoholic liver diseases. Electronmicroscopically, the hyalins were composed of granular, or homogeneous electron dense materials with the peripheral meshwork of randomly oriented fibrils” measuring about 100A in diameter. These were stained with anticytokeratin antibodies, suggesting the relateness of the hyalins to intermediate‐sized filaments of the cytokeratin type.

Kamiya, Masuzo; Tada, Toyohiro; Kuhara, Hajime; Kishimoto, Hidemasa; Wada, Yoshiro; Hashizume, Yoshio; Wakabayashi, Takashi

doi: 10.1111/j.1440-1827.1986.tb02232.xpmid: N/A

I‐cell disease has been reported by many authors but the electron microscopic findings have been reported only rarely. The patient under study was a female infant with a normal delivery after 38 weeks’normal intrauterine life. She showed the physical findings characteristic of I‐cell‐disease, and the diagnosis was made by the analyses of lysosomal enzymes. The child died at the age of 2 years and 3 months due to respiratory insufficiency. By electron microscopy, various‐shaped membrane‐bound vacuoles were observed in the cytoplasm of various cells such as hepatocytes, myocardial muscle cells, epithelial cells of the renal glomeruli, proximal renal tubular cells, fibroblasts, and chondrocytes. By histochemical analyses we found that these intracytoplasmic storage vacuoles contained glycosaminoglycan and proteoglycan.

Kawamura, Osamu; Ohaki, Yoshiharu; Nakatani, Yukio; Misugi, Kazuaki; Yoshimura, Hiroshi; Kobayashi, Hideo; Haraguchi, Sumio; Niimura, Ichiro

doi: 10.1111/j.1440-1827.1986.tb02233.xpmid: N/A

Two autopsy cases which showed marked depletion of the right ventricular musculature of the heart accompanied with marked infiltration of the adipose tissue were reported. The first cases was an 18‐year‐old female who died of right sided congestive heart failure after about 4‐years clinical course. The autopsy disclosed marked dilation of the right atrium and ventricle. The entire free wall of the right ventricle was markedly thin. Microscopically, most of the myocardial fibers of the right ventricle were replaced by fat and fibrous tissue. The second case, a 15‐year‐old boy, whose identical twin was previously diagnosed as arrhythmogenic right ventricular dysplasia designated by Fontaineet al., died suddenly during exercise. He showed no cardiac symptoms but electrocardiogram was abnormal. Autopsy revealed majority of the myocardial fibers of the right ventricular free wall were replaced by fatty tissue. In both cases, fatty infiltration was mainly noticed at the epicardial side and some myocardial fibers remained in the fatty tissue showed hypertrophic and/or degenerative changes. Review of the literature on similar cases showing depletion of the right ventricular musculature including so‐called adult's Uhl anomaly, ARVD and dilated right ventricular myocardiopathy was conducted and the relationship of the present cases with these lesions was discussed.

Akamatsu, Taiji; Honda, Takayuki; Nakayama, Jun; Nakamura, Yoshiyuki; Katsuyama, Tsutomu

doi: 10.1111/j.1440-1827.1986.tb02234.xpmid: 3028038

A case of primary adenoid cystic carcinoma of the esophagus was reported. A 54‐year‐old male patient had a tumor in the middle third of the esophagus. Barium swallow and endoscopic examination showed a protruding tumor with a shallow ulceration in its vertex. Histological examination of the surgically removed material revealed that it was largely confined to the submucosal layer, with no metastasis to lymph nodes. The tumor cells presented three distinct patterns globular nest with irregular cystic spaces, trabecular nest, and true tubule. Electron microscopic study revealed no microvilli or triad along cystic spaces in the globular nests. Histochemical study disclosed that cystic spaces and interstitium were rich in glycosaminoglycans. The luminal surface of the true tubules of the tumor, on the other hand, was characteristically lined by sialomucins. The trabecular type‐nests differed from the globular nests in showing GSA‐II reactivity and containing abundant retinol‐binding protein. These results indicate that the three patterns represented different differentiations of the tumor cells. Twenty‐three cases of esophageal adenoid cystic carcinoma were found in the literature from 1950 to 1983 and discussed collectively.