Stem Cells

Harris, Gary J.; Zeagler, Jeanette; Hofl, Daniel D.

doi: 10.1002/stem.5530030501pmid: 4045256

Abstract Scanning electron microscopy (SEM) is a valuable tool for examining cell surface morphology and cell-cell interactions. We used SEM to study 38 patients' tumors, representing 16 histological malignancies, growing in soft agar. Using our method, we obtained high quality micrographs without residual agar or preparation artifacts. We present our method for obtaining high quality SEM of tumor colonies growing in soft agar, which provides micrographs free of debris and necrotic host tissue. Scanning electron microscopy, Clones, Human tumors, Micrographs References 1 porter K . Prescott D, Frye J. Changes in surface morphology of Chinese hamster ovary cells during the cell cycle . J Cell Biol 1973 ; 57 . 815 – 836 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Sauk Jr JJ , White JG, Whitkop Jr CJ. Alteration in the surface morphology of synchronized B-16 melanoma cells during the cell cycle . Acta Derm Venereol (Stock) 1975 ; 55 : 331 – 336 . Google Scholar OpenURL Placeholder Text WorldCat 3 Ohnishi R . Dynamics of cultured L cells as studied by cinemicroscopy and scanning electron microscopy. In: Tanaka F, ed. Scanning electron microscopy in cell biology and medicine . Amsterdam : Excerpta Medica , 1981 : 1 – 12 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 4 Fishman P , Djaldetti M, Pluznik DH. Scanning electron microscope observations of surface changes occurring in murine granulocytes and macrophages during maturation in soft agar cultures . Exp Hematol 1981 ; 9 (suppl 6 ): 628 – 636 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Molday RS , Maher P. A review of cell surface markers and labeling techniques for scanning electron microscopy . Histochem J 1980 ; 12 : 273 – 315 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Molday R . Labeling of cell surface lectin receptors for scanning electron microscopy. In: Tanaka F, ed. Scanning electron microscopy in cell biology and medicine . Amsterdam : Excerpta Medica , 1981 ; 23 – 29 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 7 Kumon H . T-4 bacteriophage as a marker for Scanning electron microscopy. In: Tanaka F. ed. Scanning electron microscopy in cell biology and medicine . Amsterdam : Excerpta Medica , 1981 ; 41 – 48 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 8 Cottler-Fox M , Ryd W, Hagmar B, Fox CH. Adhesion of metastatic and non-metastatic carcinoma cells to glass surfaces . Int J Cancer 1980 ; 26 : 689 – 694 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Hamburger A , Salmon SE. Primary bioassay of human myeloma stem cells . J Clin Invest 1977 ; 6 : 846 – 854 . Google Scholar OpenURL Placeholder Text WorldCat 10 Hamburger A , Salmon SE. Primary bioassay of human tumor stem cells . Science 1977 ; 197 : 461 – 463 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Salmon SE , Buick RN. Preparation of permanent slides of intact soft-agar colony cultures of hematopoietic and tumor stem cells . Cancer Res 1979 ; 39 : 1133 – 1136 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 12 Bell Jr PB , Revel J-P. Scanning electron microscope applications to cells and tissues in culture. In: Hodges GM, ed. Biomedical research applications of scanning electron microscopy . London : Academic Press , 1980 :. 1 – 63 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 13 Bqde A , Bailey E, Jones SJ, Iitmarin A. Dimensional changes during specimen preparation for scanning electron microscopy . Scanning Electron Microscopy 1977 ; 1 : 507 – 518 . Google Scholar OpenURL Placeholder Text WorldCat 14 Rubin RW , Everhart LP. The effect of cell-to-cell contact on the surface morphology of Chinese hamster wary cells . J Cell Biol 1973 ; 57 : 837 – 844 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Takahashi, Masuhiro; Fujiwam, Masahiro; Kishi, Kenji; Sakai, Chikara; Sad, Masayoshi; Moriyama, Yoshiaki; Shibata, Akira

doi: 10.1002/stem.5530030502pmid: 3900241

Abstract Due to an increase in mature neutrophils, a 72-year-old female with gall bladder cancer showed leukocytosis of up to 1.32 × 1011/I; hypercalcemia was up to 7.7 mEq/1 in her terminal stage. Leukocyte counts and calcium values increased as the tumor progressed. There was no sign of infection or bone marrow metastasis. Cultured cells from the tumor tissue produced high colony-stimulating factor (CSF) activity into the supernatant. The tumor cell-conditioned medium stimulated exclusively granulocytic colonies. The study of this patient shows that leukocytosis was caused by the CSF produced by tumor cells. Approximately 90% of CSF activity was lost by heat treatment at 60°C for 30 min. The CSF was stable Over a pH range of 3-11 and was inactivated by treatment with proteolytic enzymes, but was not affected by treatment with DNase or neuraminidase. Molecular weight of the CSF, demonstrated by fractionation using Sephacryl S-200, was approximately 27,000 to 30.000. Gall bladder cancer, Leukocytosis, Hypercalcemia, Colony-stimulating factor (CSF) References 1 Robinson WA . Granulocytosis in neoplasia . Ann NY Acad Sci 1974 ; 230 : 212 – 218 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Asano S , Urabe A, Okabe T, et al. Demonstration of granulopoietic factor(s) on the plasma of nude mice transplanted with a human lung cancer and in the tumor tissue . Blood 1977 ; 49 : 845 – 852 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Kimura N , Niho Y, Ono J, Miyamoto N, Shibuya T, Takaki R. An established lung cancer cell line producing colony-stimulating activity . Proc Japan Acad 1978 ; 54 : 548 – 552 . Google Scholar Crossref Search ADS WorldCat 4 Sato N , Asano S, Ueyama Y, et al. Granulocytosis and colony-stimulating activity (CSA) produced by a human squamous cell carcinoma . Cancer 1979 ; 43 : 605 – 610 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Wu M , Cini JK, Yunis AA. Purification of a colony stimulating factor from cultured pancreatic carcinoma cells . J Biol Chem 1979 ; 254 : 6226 – 6228 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Svet-Moldavsky GJ , Zinzar SN, Svet-Moldavskaya IA, et al. CSF-producing human tumor cell lines. Lack of CSF-activity of human stromal bone marrow fibroblasts . Exp Hematol 1980 ; 8 (suppl 7 ): 133 . Google Scholar OpenURL Placeholder Text WorldCat 7 Di Persio JF , Brennan JK, Lichtman MA, Speiser BL. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species . Blood 1978 ; 51 : 501 – 519 . Google Scholar Crossref Search ADS WorldCat 8 Golde DW , Quan SG, Cline MJ. Human T-lymphocyte cell line producing colony-stimulating activity . Blood 1978 ; 52 : 1068 – 1072 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Hocking W , Goodman J, Golde D. Granulocytosis associated with tumor cell production of colony-stimulating activity . Blood 1983 ; 61 : 600 – 603 . Google Scholar Crossref Search ADS PubMed WorldCat 10 husis AJ , Quon DH, Golde DW. Purification and characterization of a human T-lymphocyte-derived granulocyte-macrophage colony-stimulating factor . Blood 1981 ; 57 : 13 – 21 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 11 Fojo SS , Wu MC, Gross MA, Purcell Y, Yunis AA. Purification and characterization of a colony stimulating factor from human lung . Biochemistry 1978 ; 17 : 3109 – 3116 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Nicola NA , Metcalf D, Johnson GR, Burgess AW. Preparation of colony stimulating factors from human placental conditioned medium . Leukemia Res 1978 ; 2 : 313 – 322 . Google Scholar Crossref Search ADS WorldCat 13 Price GB , Send JS, McCulloch EA, Till JE. The isolation and properties of granu-locytic colony-stimulation activities from medium conditioned by human peripheral leukocytes . Biochem J 1975 ; 148 : 209 – 217 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Hosoi, Takayuki; Ozawa, Keiya; Urabe, Akio; Takaku, Fumimaro

doi: 10.1002/stem.5530030503pmid: 3930631

Abstract We have examined the effects of recombinant immune and leukocyte interferons (rIFN-γ and rIFN-a) on the clonogenic growth of leukemic cells and normal hemopoietic progenitors using in vitro colony assays. Both interferons suppressed the colony formation by granulocyte-macrophage progenitors (CFU-gm) and erythroid progenitors (CFU-e and BFU-e) in a dose-dependent manner. Six myeloid leukemic cell lines were less sensitive to rIFN-γ than CFU-gm. The colony formation of some myeloid leukemic cell lines was suppressed more potently by rIFN-α than by CFU-gm. Four lymphoid leukemic cell lines of the T-cell type were very resistant to both recombinant interferons. Reduced sensitivity of leukemic cells to rIFN-γ, a possible hemopoietic regulator, may explain partially the unregulated proliferation of leukemic cells in vivo. Recombinant interferons, Hemopoietic progenitors, Leukemic cell proliferation References 1 Balkwill FR , Oliver RTD. Gtawth inhibitory effects of interferon on normal and malignant human hemopoietic cells . Int J Cancer 1977 ; 20 : 500 – 505 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Grant S , Bhalla K. Differential effect of recombinant human leukocyte interferon on human leukemic and normal myeloid progenitor cells . Biochem Biophys Res Comm 1980 ; 108 : 1048 – 1055 . Google Scholar Crossref Search ADS WorldCat 3 Broxmeyer HE , Lu L, Plazer E, Feit C, Juliano L, Rubin BY. Comparative analysis of the influences of human gamma, alpha and beta interferons on human multipotential (CFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells . J Immunol 1983 ; 131 : 1300 – 1305 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 4 Merigan T . Pharmacokinetics and side effects of interferon in man . Texas Rep Biol Med 1977 35 541 – 447 . Google Scholar OpenURL Placeholder Text WorldCat 5 Zoumbos N , Gascon P, Young N. The function of lymphocytes in normal and suppressed hematopoiesis . Blut 1984 ; 48 : 1 – 9 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Lozzio CB , Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome . Blood 1975 ; 45 : 321 – 334 , Google Scholar Crossref Search ADS PubMed WorldCat 7 Koeffler HP , Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity . Science 1978 ; 200 : 1153 – 1154 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Minowada J . Immunology of leukemic cells. In: Gum , Hendersons, eds. Leukemia . 4th ed. New York : Grune and Stratton , 1982 : 119 – 139 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 9 Collins SJ , Gallo RC, Gallagher RE. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture . Nature 1977 ; 270 : 347 – 349 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Sundstrom C , Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937) . Int J Cancer 1976 ; 17 ; 565 – 577 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Tsuchiya S , Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T. Establishment and characterization of a human acute monocytic leukemia cell line (THP-I) . Int J Cancer 1980 ; 26 : 171 – 176 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Minowada J , Ohnuma T, Moore GE. Brief Gommunication: Rosette forming lymphoid cell line I. Establishment and evidence for origin of thymus-derived lymphocytes . J Natl Cancer Inst 1972 ; 49 : 891 – 895 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 13 Lazarus H , Barell EF, Oppenheim S, Krishan A. Divergent properties of two lympho-cytic cell lines isolated from a single specimen of peripheral blood . In Vitro 1974 ; 9 : 303 – 330 . Google Scholar Crossref Search ADS WorldCat 14 Foley GE , Lazarus H, Farber S, Uzman BG, Boone BA, McCarthy RE. Continuous culture of human lymphoblasts from peripheral blood of achild with acute leukemia . Cancer 1965 ; 18 : 522 – 529 . Google Scholar Crossref Search ADS PubMed WorldCat 15 Robinson W , Metcalf D, Bradley TR. Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells . J Cell Physiol 1969 ; 69 : 83 – 92 . Google Scholar Crossref Search ADS WorldCat 16 Iscove NN , Sieber F, Winterhalter KH. Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoiesis by gel filtration and affinity chromatography on agarose-concanavalin A . J Cell Physiol 1974 ; 83 : 309 – 320 . Google Scholar Crossref Search ADS PubMed WorldCat 17 Blalock JE , Georgiades JA, Langford MP, Johnson HM. Purified human immune interferon has more potent anticellular activity than fibroblast or leukocyte interferon . Cell Immunol 1980 ; 49 : 390 – 394 . 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Erythropoietin-independent stimulation of early erythropoiesis in adult marrow cultures by conditioned media from lectin stimulated mouse spleen cells. In: Golde , Cline, eds. Hematopoietic cell differentiation . New York : Academic Press , 1983 52 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 23 Branca AA , Conado B. Evidence that types I and II interferons have different receptors . Nature 1980 ; 294 : 768 – 770 . Google Scholar Crossref Search ADS WorldCat 24 Sarkar FH , Gupta SL. Receptors for human γ-interferon: binding and crosslinking of 125I-labeled recombinant human γ-interferon to receptors on WISH cells . Proc Natl Acad Sci USA 1984 ; 81 : 5160 – 5164 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Shimamura, Michiya; Urnbe, Akio; Takaku, Fumimaro; Mizuno, Masao

doi: 10.1002/stem.5530030504pmid: 4045257

Abstract The exogenous addition of dolichyl phosphate (Dol-P), an active form of dolichol pol) that carries oligosaccharide chains for protein-N-glycosylation, significantly enhanced colony formation of mouse bone marmw hematopietic pmgenitors (CFUe, BFUe, and CFU-gm) was stimulated by erythropietin (Epo) and colony-stimulating factor (CSF), but Do1 enhanced colony formation of CFUe only. The effects of Do1 or Dol-P on these hematopoietic progenitors were fully dependent on stimulation by Epo or CSF. Other mevalonate-metabolites, such as cholesterol, coenzyme Q10, and isopentenyladenine, had no effect on hematopoietic progenitors. These studies suggest that exogenous Dol-P enhances the frequency of differentiation of hematopoietic progenitors stimulated by Epo or CSF, and there may be a diversity in cellular response of these progenitors to Dol. Dolichol Dolichyl phosphate, Differentiation CFU-e, BFU-e, CFU-gm References 1 Waechter CJ , Lennarz WJ. The role of polyprenol-linked sugars in glycoprotein synthesis . Ann Rev Biochem 1976 ; 45 : 95 – 112 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Carson DD , Lennarz WJ. Relationship of dolichol synthesis to glycoprotein synthesis during embryonic development . J Biol Chem 1981 ; 256 : 4679 – 4686 . Google Scholar Crossref Search ADS PubMed WorldCat 3 James MJ , Kandutsch AA. Elevated dolichol synthesis in mouse testes during spermato-genesis . J Biol Chem 1980 ; 255 : 16 – 19 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Potter JER , James MJ, Kandutsch AA. Sequential cycles of cholesterol and dolichol synthesis in mouse spleens during phenylhydrazine-induced erythropoiesis . J Biol Chem 1981 ; 256 : 2371 – 2376 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Potter JER , Kandutsch AA. Increased synthesis and concentration of dolichyl phosphate in mouse spleens during phenylhydrazine-induced erythropoiesis . Biochem Biophys Res Commun 1982 ; 106 : 691 – 696 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Hoffman PC , Richman CM, Larson RA, Yachnin S. Cholesterol and mevalonic acid are independent requirements for the in vitro proliferation of human bone marrow granulocyte progenitor cells: studies using ML236B . Blood 1983 ; 61 : 667 – 671 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Endo A . 3-Hydroxy-3-methylglutaryl-Co A reductase inhibitors from Penicillium and Monuscus species. In: Lowenstein, eds. Methods in enzymology . New York : Academic Press , 1981 ; 2 : 684 – 689 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 8 Suzuki S , Mori F, Takigawa T, Ibata K, Ninagawa Y, Nishida T, Mizuno M, Tanaka Y. Synthesis of mammalian dolichols from plant polyprenols . Tetrahedron Lett 1983 ; 24 : 5103 – 5106 . Google Scholar Crossref Search ADS WorldCat 9 Danilov LL , Chojnacki T. A simple procedure for preparing dolichyl monophosphate by use of POC13 . FEBS Lett 1981 ; 131 : 310 – 312 . Google Scholar Crossref Search ADS WorldCat 10 Heifetz A , Lennarz WJ. Biosynthesis of N-glycosidically linked glycoproteins during gastrulation of sea urchin embryos . J Biol Chem 1979 ; 254 : 6119 – 6127 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Carson DD , Lennarz WJ. Inhibition of poly isoprenoid and glycoprotein biosynthesis causes abnormal embryonic development . Proc Natl Acad Sci USA 1979 ; 76 : 5709 – 5713 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Harford JB , Waechter CJ. Effect of exogenous dolichyl monophosphate on a developmental change in mannosylphosphoryldolichol biosynthesis . Biochem Biophys Res Commun 1977 ; 76 : 1036 – 1043 . Google Scholar Crossref Search ADS PubMed WorldCat 13 Harford JB , Waechter CJ. A developmental change in dolichyl phosphate mannose synthase activity in pig brain . Biochem J 1980 ; 188 : 481 – 490 . Google Scholar Crossref Search ADS PubMed WorldCat 14 Burton WA , Scher MG, Waechter CJ. Enzymatic phosphorylation of dolichol in central nervous tissue . J Biol Chem 3979 ; 254 : 7129 – 7136 . Google Scholar Crossref Search ADS WorldCat This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Knospe, William H.; Husseini, Salah G.; Adler, Solomon S.; Reddi, A. Hari

doi: 10.1002/stem.5530030505pmid: 4045258

Abstract Implants of bone and tooth matrix powder were placed subcutaneously (s.c.) on intrapentoned (i.p.) Mitex or Fblyvic membranes. Implants were removed for histology after 1-24 weeks. Macrophages, fibroblasts, and vascular sinusoids infiltrated around bone and tooth matrix particles after one week. In the S.C. tooth matrix implants, a few sites of cartilage formation and ossification developed at two weeks, and by three weeks granulocytopoiesis and megakaryaytopoiesis developed adjacent to new bone; erythro-poiesis was not observed. In S.C. bone matrix implants and in the i.p. artificial membranes coated with bone or tooth matrix powder, ossification or hematopoiesis was not observed. Small numbers of CFU-s, CFU-nm, BFU-e, and CFU-e appeared 10-20 days after S.C. implantation of tooth matrix; none were detected in S.C. bone matrix implants. Tooth matrix powder, Hematopoietic microenvironment References 1 Urist MR . Bone formation by autoinduction . Science 1965 ; 150 : 893 – 899 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Reddi AH , Huggins CB. Formation of bone marrow in fibroblast-transformation ossicles . Proc Natl Acad Sci USA 1975 ; 72 : 2212 – 2216 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Huggins CB , Urist MR. Dentin matrix transformation: rapid induction of alkaline phosphatase and cartilage . Science 1970 ; 167 . 896 – 898 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Reddi AH . The matrix of rat calvarium as transformant of fibroblasts . Proc Soc Exp Biol Med 1975 ; 150 : 324 – 326 . Google Scholar Crossref Search ADS PubMed WorldCat 5 Reddi AH , Anderson WA. Collagenous bone matrix-induced endochondral ossification and hemopoiesis . J Cell Biol 1976 ; 69 : 557 – 572 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Huggins CB , Wiseman S, Reddi AH. Transformation of fibroblasts by allogeneic and xenogeneic transplants of demineralized tooth and bone . J Exp Med 1970 ; 132 : 1250 – 1258 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Knospe WH , Husseini SG, Adler SS, Reddi AH. Hematopoiesis on cellulose ester membranes (CEM). V. Enrichment of CEM by demineralized mouse bone matrix powder . Exp Hematol 1983 ; 11 : 1021 – 1026 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 8 Knospe WH , Husseini SG, Adler SS. Hematopoiesis on cellulose ester membranes (CEM). IV. Effect of variation in membrane composition and pore size . Int J Cell Cloning 1984 ; 2 : 99 – 112 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Reddi AH , Huggins CB. Biochemical sequences in the transformation of normal fibroblasts in adolescent rats . Proc Natl Acad Sci USA 1972 ; 69 : 1601 – 1605 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Till JE , McCulloch EA. A direct measuwment of the radiation sensitivity of normal mouse bone marrow cells . Radiat Res 1961 ; 14 : 213 – 222 . 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New York : Springer-Verlag , 1978 ; 257 – 261 . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC 15 Knospe WH , Husseini S, Trobaugh FE Jr. Hematopoiesis on cellulose ester mern- branes (CEM). II. Enrichment of the hematopoietic microenvironment by the addi- tion of selected cellular elements . Exp Hematol 1978 ; 6 : 601 – 612 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 16 Knospe WH , Husseini SG, Adler SS. Hematopoiesis on cellulose ester membranes (CEM). III. Long term histologic changes between 6 and 12 months after i.p. irn- plantation . Exp Hematol 1983 ; 11 : 512 – 521 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 17 Taivassoli M , Crosby WH. Transplantation of marrow to extramedullary sites . Science 1968 ; 161 : 54 – 56 . Google Scholar Crossref Search ADS PubMed WorldCat 18 Knospe WH , Adler SS, Husseini SG. Hematopoiesis on cellulose ester membranes (CEM). IX. Effect of irradiation on stromal cell enrichment and enrichment of CEM with matrix proteins alone [Abstract] . Exp Hematol 1984 ; 12 : 417 . Google Scholar OpenURL Placeholder Text WorldCat 19 McCuskey RS , Meineke HA, Pinkstaff CA, Knospe WH. Hematopoiesis on cellulose ester membranes (CEM). VI. Histochemical evaluation of stromal-CEM interactions after strornal enrichment . Exp Hematol 1984 ; 12 : 25 – 30 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 20 Spooncer E , Gallagher JT, Dexter TM. The role of the cellular matrix in haemopoiesis [Abstract] . Exp Hematol 1982 ; 10 (suppl 11 ): 68 . Google Scholar OpenURL Placeholder Text WorldCat 21 Robinson HC , Brett MJ, Tralaggan PJ, Lowther DA, Okayarna M. The effect of D-xylose, 8-D-xylosides and 8-D-galactosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage . Biochem J 1975 ; 148 : 25 – 34 . Google Scholar Crossref Search ADS PubMed WorldCat 22 Zuckerman KS , Wicha MS. Extracellular matrix production by the adherent cells of long-term murine bone marrow cultures . Blood 1983 ; 61 : 540 – 547 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Reddi AH . Collagen and cell differentiation. In: Ramachandran GN, Reddi AH, eds. Biochemistry of Collagen . New York : Plenum Publishing Corp , 1976 : 449 – 478 Google Scholar Crossref Search ADS Google Preview WorldCat COPAC 24 Kubolci Y , Mechanic GL. Comparative molecular distribution of cross-links in bone and dentine collagen structure, function'relationships . Calcif Tissue Int 1982 ; 34 : 306 – 308 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 25 Seed TM , Husseini SG, Adler SS, Knospe WH. Hematopoiesis on cellulose ester membranes (CEM). VII. Ultrastructure of stroma of marrow-enriched membranes with trilineal hematopoiesis [Abstract] . Exp Hematol 1983 ; 11 (suppl 14 ): 145 . Google Scholar OpenURL Placeholder Text WorldCat 26 Dexter TM , Testa NG, Allen TD, Rutherford T, Scolnick E. Molecular and cell biologic aspects of erythropoiesis in long-term bone marrow cultures . Blood 1981 ; 58 : 699 Google Scholar Crossref Search ADS PubMed WorldCat 27 W. N. Bermani , Newby El. Erythropoietic response of bone marrow cells cultivated in dif- fusion chambers . Nature 1967 ; 213 : 300 – 301 . Google Scholar Crossref Search ADS WorldCat 28 Lowry PH , Keighley G. Inactivation of erythropoietin by Wand's tryptophan reagent and by membrane filtration . Biochem Biophys Acta 1968 ; 160 : 413 – 419 . Google Scholar PubMed OpenURL Placeholder Text WorldCat This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Astaldi, G.; FoÁ, R.; Ghio, R.; Neri, A.; Pistoia, K

doi: 10.1002/stem.5530030506pmid: 3876398

Abstract We report on the main topics discussed at the “Human T-Lymphocytes in Normal and Leukemia Patients” meeting, including the “Second International Workshop on T-cell Colonies” (Reggio Calabria, September 16-19, 1984). Improved knowledge in the field of T-lymphocyte biology has contributed greatly to a better clinical definition of T-cell-derived lymphoproliferative disorders. The T-cell colony assay seems to represent a useful tool for studying the early steps of T-lymphocyte maturation and for characterizing accessory cells and soluble mediators that regulate T-cell proliferation. T-lymphocytes, T-cell colonies, T-cell leukemia References 1 Donnenberg AD , Noga SJ, Schwartz CL, Santos GW. Isolation of lymphoid and pre-lymphoid colony precursors in human bone marrow (BM) fractionated by counterflow centrifugation elutriation (CCE). 2 Mossallay AM , Goube de Laforest P, Tanzer J. T cell colony formation: a reliable assay detecting pre-T-cells in human bone marrow . 3 Knott L , Linch DC. Bone marrow derived T-cell colony forming cells: their use in the assessment of T-cell purging . 4 Farcet JP , Gourdin MF, Oudrhiri N, Calvo CS, Divine M, Bouguet J, Fradelizi D, Senik A, Reyes F. Heterogeneity of human T-lymphocyte: a subset of OKT4+ penpheral T cells can generate colonies containing mixed progeny with OKT4+‘ and OKT8+’ suppressor cells . 5 Goldman J , Poran H, Werber MN, Shoham D, Radnay J. TWO distinct interleukins promote growth of the two types of T-cell colonies in soft agar culture . 6 Pistoia V , Ghio R, Ferrarini M. Accessory role of dendritic cells in the growth of pokeweed mitogen (PWM)-induced T cell colonies . 7 Martinoli C , Pistoia V, Zaccheo D. Human placental Hofbauer cells serve as accessory cells for PHA-induced T cell colony formation . 8 Schenone A , Ghio R, Pistoia V, Detotero D, Boccaccio P. In vivo and in vitro effects of antilymphocyte globulin (ALG) on T cell colony growth . 9 Lusso P , Ferrando ML, Fierro MT, Giubellino MC. Foá R. Effect of exogenous inter-leukin-2 on the growth of T-colonies in B-chronic lymphocytic leukemia . 10 Lunardi-Iskandar Y , Georgoulias V, Allouche M, Klatzman D, Cavaille Coll M, Mayer P, Rozenbaum W, Gluckman JC, Jasmin C. Abnormal in vitro proliferation and differentiation of T-colony forming cells in AIDS patients . 11 Georgoulias V , Triebel F, Kosmatopoulos C, Allouche M, Gluckman JC, Jasmin C. Spontaneous activation and constitutive pduction of T cell growth factors by leukemic T cells in human T cell malignancies . 12 Ennis KT , Irvine AE, Morris TCM, Alexander HD. T-lymphocyte colony formation; interactions between normal donors and patients with aplastic anemia . 13 Morabito F , Liotta C, Neri A. T-colonies in non-Hodgkin's lymphoma: inhibitory effect of adherent cells and growth restoration by indornethacin . 14 Jmni P , Corvetta A, Nasia C, Montillo M, Centunoi R. T-cell colonies in rheumatoid arthritis . 15 Osculati F , Balercia G, Cinti S, Dantchev D, Mathè G. An immunocytochemical ultra-structural study on normal human thymocytes at discrete stages of differentiation . 16 Larocca LM , Musiani P, Piantelli M, Leone G. Human thymoma: a model for the study of T-lymphocyte differentiation . 17 Holter W , Majdic O, Stockinger H, Liszka K, Koller U, Knapp W. Changing phenotype of human T cells during differentiation and activation . 18 Bonnefoix T , Sotto JJ. Regulating factors produced by T subset populations on human granulopoiesis in vitro . 19 Douer D , Froom P, Shaked N, Biniaminov M, Ramot B. Production of burst promoting activity by malignant T-lymphocytes arrested at early stages of T-cell differentiation . 20 Pegoraro L , Ilusso P, Cordero di Montezemolo L, Ramello A, Fierro MT, Matera L, Foi R. Establishment of a human leukemic cell line (PF-382) with phenotypic and functional features of suppressor T-lymphocytes . 21 Martinotti MG , Foá R, Martino F, Jemma C, Fierro MT, Forni G. Growth in nude mice of a T-cell line (PF-382) from a case of T-acute lymphoblastic leukaemia: a possible model for experimental immunotherapy . 22 Donelli A , Ferrari S, Emilia S, Selleri L, Venturelli D, Moretti L, Ceccherelli G, De Benedittis MG, Zucchini P, Torelli G, Torelli U. Expression of c-myc onc-gene in total cellular RNA of human normal and leukemic T cells and in human normal bone marrow cells . 23 Matutes E , Catovsky D. Ultrastructural features of T-cell leukaemias . 24 Crockard AD , Macfarlane E, Andrews C, San Miguel GF, Bridges JM, Catovsky D. Cytochemical characteristics of leukaemic T-cells: correlation of hydrolytic enzyme reactivities with membrane phenotypes . 25 Liso V , Riezzo A, Specchia G, Pavone V, Santorsola D, Mannarella C, Magno M, Ferrante R. Cytology-cytochemistry of acute lymphoblastic leukaemia of T-type (T-ALL). Usefulness of some cytochemical patterns . 26 Pandolfi F , De Rossi G, Semenzato G, Ranucci A, Bonomo G, Napolitano M, Cafam A, Mezzarima I, Lucian M. Abnormal expansion of granular lymphocytes . 27 Lauria F , Raspadori D, Tauari PL, Motta MR, Foi R, Biagini G, Preda P, Tura S. Phenotypic heterogeneity and functional behaviour of T-cell chronic malignancies . 28 Mathk G , Balercia G, Dantchev D, Lemaigre G, Roulon A, Bignon YJ. T-lymphoid leukemia-lymphoma categorization . 29 Guglielmi C , De Rossi G, Bpez M, Martelli M, Pasqualetti D, Pezzella F, Stoppacciaro A, Mandelli F. 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A lymphocyte subpopulation within the T-helper subset exhibits phenotypic and developmental characteristics of NK cells . 38 De Felice L , Arcese W, Lopez M, Mauro FR, Torti F, Bianchi A, Riguzzi C, Guglielmi C, Papa G. Sequential study of bone marrow and peripheral blood CFU-gm growth and lymphocyte subpopulations in SAA patients treated with combined ISS . 39 Velardi A , Grossi CE. Lymphocytes with T-helper phenotype in B-cell malignancies . 40 Foá R , Giovarelli M, Jemma C, Lusso P, Fierro MT, Ferrando ML, Lauria F, Forni G. Interleukin-2 and interferon-production by a B-CLL T-lymphocytes . 41 Jaksic B , Vitale B, Burek B, Benkwic B, Antica M, Hauptmann E. In vitro and in vivo modulation of T-cell function in B-cell chronic lymphocytic leukemia . 42 Brugiatelli M , Callea V, Neri A. T-cell subset in B-chronic lymphocytic leukaemia (B-CLL) patients in remission phase . 43 Worman CP , Camba L, Joyner M, Toy JL, Cawley JC. Interferon in hairy cell leukaemia: effect on hairy cells, T cells and normal haematopoiesis . This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Bosanquet, Andrew G.

doi: 10.1002/stem.5530030507pmid: 4045259

References 1 Franco R , Kraft T, Miller T, Fbpp M, Martelo O. Storage of chemotherapy drugs for use in the human tumor stem cell assay . Int J Cell Cloning 1984 ; 2 : 2 – 8 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Bird MC , Bosanquet AG, Gilby ED. In vitro determination of tumour chemosensitivity in haematological malignancies . Hematol Oncol (in press). 3 Salmon SE . Human tumor colony assay and chemosensitivity testing . Cancer Treat Rep 1984 ; 68 : 117 – 125 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 4 Von Hoff DD , Clark GM, Stogdill BJ, et al. Prospective clinical trial of a human tumor cloning system . Cancer Res 1983 ; 43 : 1926 – 1931 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Weisentdal LM , Marsden JA, Dill PL, Macaluso CK. A novel dye exclusion method of testing in vitro chemosensitivity of human tumors . Cancer Res 1983 ; 43 : 749 – 757 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 6 Bosanquet AG . Stability of solutions of antineoplastic agents during preparation and storage for in vitro assays . I. General considerations, the nitrosoureas and alkylating agents. Cancer Chemother Pharmacol (in press). 7 Trissel LA . Avoiding common flaws in stability and compatibility studies of injectable drugs . Am J Hosp Pharm 1983 ; 40 : 1159 – 1160 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 8 Flora KP , Smith SL, Cradock JC. Application of a simple high-performance liquid chromatographic method for determination of melphalan in the presence of its hydrolysis products . J Chromatogr 1979 ; 177 , 91 – 97 . Google Scholar Crossref Search ADS PubMed WorldCat 9 Wheeler GP . A review of studies on he mechanism of action of nitrosoureas . Amer Chem Soc Symp Series 1976 ; 30 : 87 – 119 . Google Scholar OpenURL Placeholder Text WorldCat 10 Bosanquet AG . Stability of solutions of melphalan during preparation and storage for in vitro chemosensitivity assays . J Pharm Sci (in press). 11 Yang LY , Drewinko B. The stability of the lethal efficacy of antitumor drugs . Proc Am Assoc Cancer Res 1983 ; 24 : 315 . Google Scholar OpenURL Placeholder Text WorldCat Article PDF first page preview Close This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Franco, Robert; Kraft, Toni; Miller, Timothy; Popp, Martin; Martelo, Orlando

doi: 10.1002/stem.5530030508pmid: N/A

References 1 Franco R , Kraft T, Miller T, Popp M, Martelo OJ. Storage of chemotherapy drugs for use in the human tumor stem cell assay . Int J Cell Cloning 1984 ; 2 : 2 – 8 . Google Scholar Crossref Search ADS PubMed WorldCat 2 Trissel LA . Avoiding common flaws in stability and compatibility studies of injec-table drugs . Am J Hosp Pharm 1983 ; 40 : 1159 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Article PDF first page preview Close This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

doi: 10.1002/stem.5530030509pmid: N/A

Article PDF first page preview Close This content is only available as a PDF. © 1985 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)