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Alumets, J.; Ekelund, G.; Håkanson, R.; Ljungberg, O.; Ljungqvist, U.; Sundler, F.; Tibblin, S.
doi: 10.1007/BF00427182pmid: 148774
A case of malignant endocrine tumour of the jejunum, associated with severe duodenal ulcer is described. The tumour and a local metastasis were examined by immunohistochemistry and found to contain abundant somatostatin-immunoreactive cells together with less numerous cells displaying gastrin immunoreactivity. This is to our knowledge the first case of intestinal somatostatinoma. The presence of gastrin cells in the tumour may explain the ulcer diathesis.
doi: 10.1007/BF00427183pmid: 207015
The aim of this study was to investigate the qualitative and quantitative changes of ACTH-cells in the rat after application of a specific and a non-specific stimulus. A CRF-analog (lysin-vasopressin) and a prostaglandin (prostaglandin E1) were used. 40 rats were injected lysin-vasopressin or prostaglandin E1, respectively, for 4 weeks. The pituitary glands were investigated by means of light microscopy, electron microscopy and morphometry. Activation of the ACTH-cells could be observed after use of both substances, the effect of lysin-vasopressin being more intense than that of prostaglandin E1. Enlargement of the nucleus, the cytoplasm and the organelles involved in hormone-production and -transport were found and verified by morphometry. Additionally an increase in number of the cells could be demonstrated. Prostaglandin influenced not only ACTH-cells, but also other cells of the anterior pituitary.
Dyer, Robert; Peppler, Richard
doi: 10.1007/BF00427184pmid: 148775
Copper wire was inserted into one uterine horn of nulliparous female rats, and remained in place until the animals were killed 1 month or 3 months post-insertion. The opposite horn and uteri of non-IUD exposed rats served as control tissue. Light microscopy of the horn that contained the device revealed distortion of the uterine lumen, leucocyte infiltration, and patchy metaplasia of the simple columnar endometrial epithelium into a pseudostratified or stratified squamous epithelium with occasional keratinization. Electron microscopy demonstrated that the stratified squamous epithelium was typical of that found normally at other sites. The cells were attached by numerous desmosomes to one another, and hemidesmosomes anchored the basal cells to the basal lamina. The cells contained numerous bundles of tonofilaments, and keratohyalin granules were present where keratinization occurred. Membrane-coating granules were seen within and between cells of the upper layer. The results of the study indicate that the copper IUD alters the uterine lumen and epithelium in a manner similar to inert devices. It would appear that these changes could interfere dramatically with blastocyst implantation. This effect, along with the known reduction in numbers of ovulated eggs and the possible toxic nature of this medicated device, may combine to increase dramatically the efficacy of the device in the rat.
TerÄvÄinen, Heikki; Juntunen, Juhani; Eriksson, Kalervo; Larsen, Andreo
doi: 10.1007/BF00427185pmid: 148776
Muscles of the lower legs of rats given 25% ethanol in water ad libitum for up to 9.5 months were studied using histological, histochemical and electrophysiological techniques. Ethyl alcohol was substituted for about 20% of the total calorific input of the animals. The observations were compared with the structure of the gastrocnemius muscle of five alcoholics with clinical neuropathy. Fibrillation potentials and angulated atrophic fibers were observed in the muscles of animals on alcohol for 9.5 months. No fiber type grouping was present. There was also phagocytosis of the muscle fibers and changes in their internal structure, as reflected by the distribution of NADH-diaphorase. The observed muscle changes in the alcoholics and those in the experimental animals on alcohol differed mainly quantitatively, the only exception being the presence of fiber type grouping in the biopsies from the alcoholics.
Landolt, A.; Rothenbühler, Verena
doi: 10.1007/BF00427186pmid: 148777
Ultrastructural examination of 184 pituitary adenomas demonstrated the presence of extracellular accumulations of electron dense material in 3 out of 64 cases with acromegaly. Fibrillary structures were seen in larger deposits of such material. This material was only observed in biopsies fixed directly with osmium tetroxide; initial fixation with glutaraldehyde did not retain the material and left empty spaces. Positive immuno-histochemical reaction with specific antibodies demonstrated that the extracellular material contained growth hormone (GH). The presence of this extracellular material could not be related to the age or sex of the patient nor to the duration of symptoms, size of the tumor, presence of diabetes mellitus, or concomitant secretion of prolactin. The pericapillary fibrous sheath was heavily thickened in the patient with the longest duration of symptoms, intermediate in thickness in the second and normal in the third.
Bleyl, U.; Rieger, P.; Rossner, J.
doi: 10.1007/BF00427187pmid: 148778
In states of plasmic hypercoagulability and consumption coagulopathy ethanol favours the non-enzymatic polymerization of circulating soluble fibrinogen fibrin monomer complexes (FFMC) in vitro. The ethanolgelation test of Godal and Abildgaard makes use of this phenomenon, called paracoagulation. The present studies show that it is also possible to visualize soluble FFMC by means of ethanol-gelation. In the electron microscope, FFMC, polymerized non-enzymatically by ethanol in the spleen, are characterized by plump or slender mycelioid fibrillar precipitates that show a uniform rhythmic transverse striation, a period-coincidental filamentary arrangement and an average periodicity of 23 nm. The ultrastructure demonstrates these ethanol-induced filaments to be in vitro-polymerized fibrin monomer derivatives. Paracoagulation with ethanol allows the identification of soluble FFMC in the tissue prior to the formation of highly polymerized fibrin-rich microthrombi, the established equivalents of the DIC-syndrome.
doi: 10.1007/BF00427188pmid: 207016
Electron microscopic findings are reported on the localization and fine structure of glycolipid inclusions in different organs (heart, kidney, lymph nodes, arterial blood vessels, pancreas) in Fabry's disease in a female. The intracellular and extracellular inclusions were made up of multilamellar membraneous systems in concentric, excentric, and parallel arrangement. This fine structure is characteristic of liquid-crystalline phases of phospholipid-water systems.
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