THE EFFECT OF COMMERCIAL AND PURE GENTAMICIN ON THE GROWTH OF HUMAN DIPLOID LUNG FIBROBLASTSLitwin, Jack
doi: 10.1111/j.1699-0463.1970.tb04302.xpmid: 5273515
The effect of different concentrations of pure and commercial preparations of gentamicin on the growth of human embryonic diploid lung fibroblasts was studied. The commercial preparation was slightly more stimulatory than the pure antibiotic at concentrations of 1 μg/ml and 10 μg/ml. At concentrations of 100μg/ml and 500 μg/ml the commercial preparation was toxic but the pure antibiotic still permitted growth. Cell growth was stimulated above that of the control and cell longevity in vitro increased with 10 μg/ml. Gentamicin had no effect on cell adsorption at any of the concentrations used.
EFFECT OF PRIMARY IMMUNIZATION WITH SHEEP ERYTHROCYTES ON THE RELEASE OF CELLS FROM THE SPLEEN AND ON PERIPHERAL BLOOD LYMPHOCYTE POPULATION IN YOUNG GUINEA‐PIGSSandberg, Göran
doi: 10.1111/j.1699-0463.1970.tb04303.xpmid: 5273516
Splenic cell migration was evaluated by lymphocyte counts in splenic afferent and efferent blood, the lymphocytes being divided into groups with different mitochondrial content (corresponding to the different size of the lymphocytes). The effect of a single intraperitoneal injection of sheep red blood cells (SRBC) was studied 3–15 days after the injection. Cell counts of splenic arterial blood showed a decreased percentage of lymphocytes with low mitochondrial content after injection with SRBC. Per mm3 of blood the absolute number of lymphocytes with 0–5 mitochondria (the smallest lymphocytes) was reduced up to 40 per cent 9–15 days after injection of SRBC. The number of lymphocytes with higher mitochondrial content tended to decrease earlier, and to reach a normal, or above normal, level within a few days. The normal increase in the total number of lymphocytes in growing guinea‐pigs was not affected by the treatment. No effect of SRBC on splenic cell emigration was observed for certain, although there was a possible increase 6–12 days after injection, reaching a maximum on the 6th day. Thymic weight was reduced 3 days after SRBC injection and remained somewhat lower than in control animals 9–15 days after the injection. There was no significant effect on splenic or lymph‐node weight. Initially, the normal increase in cervical lymph‐node weight was to some extent suppressed in the treated animals, however, and splenic weight tended to increase after SRBC.
ETHYLENE OXIDE RESISTANCE OF MICRO‐ORGANISMS IN DUST COMPARED WITH THE RESISTANCE OF BACILLUS SUBTILIS SPORESKristensen, Hanne
doi: 10.1111/j.1699-0463.1970.tb04306.xpmid: 4990977
The resistance of standardized spore preparations of Bacillus stearothermophilus and Bacillus subtilis to industrial ethylene oxide sterilization procedures was compared. Moreover, the resistance of the most resistant preparation was compared with that of micro‐organisms in dust and dirt. The experiments demonstrated the importance of the water content to the ethylene oxide resistance of “test pieces” intended for the control of the microbiological effect of sterilization procedures. It was demonstrated also that micro‐organisms in dust and dirt may possess an ethylene oxide resistance in the same range as that possessed by B. subtilis spores in the vacuum‐dried routine “test pieces” of Statens Seruminstitut, Copenhagen. Inactivation curves representing the subtilis strain and some of the micro‐organisms which had survived the industrial sterilization procedures revealed that the decisive factor in the inactivation was the water content inside the micro‐organisms or in their immediate vicinity.
FLUORESCENT EBV ANTIBODIES IN INFECTIOUS MONONUCLEOSISMarker, Ole
doi: 10.1111/j.1699-0463.1970.tb04307.xpmid: 4920375
The fluorescent EBV antibodies in infectious mononucleosis (IM) have been studied in 33 patients admitted to hospital with a diagnosis of IM and bled at intervals over periods from 5 to 16 weeks after onset of illness. EBV antibodies were demonstrable in all these patients. In 18 patients, maximum titres up to 2560 appeared already in the first blood specimen obtained. A slight decrease in titre was seen in these patients during the first month of illness, after which period the titres remained constant. A significant rise in antibody titre was demonstrated in sera from only 3 patients, while 13 patients showed no change in titre. The control group consisted of 24 apparently healthy individuals and 11 patients suffering from diseases other than IM. In sera from one of these patients and from 7 of the healthy persons, EBV antibodies were not demonstrable while sera from the rest of the control group showed titres ranging from 10 to 640. Studies of blood samples from 4 persons obtained over a period of 15 years show that EBV antibodies persist for this length of time without change in titre. The results show that the actual titre value of fluorescent EBV antibodies may be of very limited diagnostic significance in IM. In order to demonstrate a rise in titre it seems necessary to obtain blood specimens before or immediately after onset of illness.
ISOLATION OF LYMPHOCYTES FROM BLOOD.Tärnvik, Arne
doi: 10.1111/j.1699-0463.1970.tb04308.xpmid: N/A
Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica‐polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.
STUDIES ON THE MULTIPLICATION OF VESICULAR STOMATITIS VIRUS WITH FLUORESCEIN AND FERRITIN CONJUGATED ANTIBODIESPaucker, Kurt; Shechmeister, I. L.; Sc, A. Birch‐Andersen M.
doi: 10.1111/j.1699-0463.1970.tb04309.xpmid: 4320021
Under conditions of overwhelming infection of L and HeLa cells, with the New Jersey type of vesicular stomatitis virus (VSV), maximal infectivity titres were attained within 12 hours post‐infection. Staining with anti‐VSV fluorescent gamma globulin revealed within 4 to 6 hours after infection extensive accumulation of viral antigen in the cytoplasm. The fluorescent patches were of variable sizes and nuclei were not involved at any time. Prior to total destruction, cytoplasmic fusion occurred in L but not in HeLa cultures, resulting in the formation of multinucleated giant cells. Electron microscopy failed to detect any significant morphological changes in the cytoplasm of infected cells which might correspond to the observed immunofluorescent deposits. Efforts to locate such areas in cells rendered permeable to ferritin conjugated antibodies were unsuccessful. Within 4 to 6 hours after infection, virus accumulated at the cellular boundary or in cytoplasmic vacuoles. As a rule, only particles located at the cell periphery were readily tagged with ferritin antibody.
EFFECTS OF THYMECTOMY UPON THE FORMATION OF FÓA‐KURLOFF CELLS IN THE GUINEA PIGRanlov, Poul; Christensen, Hans Ewald; Wanstrup, John
doi: 10.1111/j.1699-0463.1970.tb04310.xpmid: 5273518
The peculiar cytoplasmic inclusion body found in mononuclear cells of guinea pig blood and lymphoid tissues—the Fóa‐Kurloff body—is believed to play an undefined role in the humoral immune mechanism of that animal. On the ultrastructural level, similarities between such cells and plasmocytic cells have earlier been noted. In order to test an often postulated thymic origin of these cells and to evaluate the effects of an immunological ablation on their formation, thymectomy was carried out on guinea pigs subsequently subjected to repeated injections of oestrogen. The experiment demonstrated unequivocally that the presence in the guinea pig of thymic tissue has no influence on the rate of formation of Fóa‐Kurloff bodies in its tissues.