journal article
LitStream Collection
Monoclonal antibodies to the hydrogenase ofThiocapsa roseopersicina
Tigyi, Gábor J.; Kovács, Kornél L.
1984 Current Microbiology
doi: 10.1007/bf01567701pmid: N/A
Monoclonal antibodies were produced to electrophoretically pure hydrogenase fromThiocapsa roseopersicina. Protein immunoelectroblotting was used to identify the hydrogenase-specific antibodies. Among the 18 monoclonal antibodies selected by enzyme immunoassay, three were found to react with highly immunogenic trace contaminating proteins. One cell line produced antibody that inhibitied hydrogenase activity. This was the first specific inhibitor of the hydrogenase function. The results suggest that monoclonal antibodies could provide valuable new informations about the enzyme structure as well.