journal article
LitStream Collection
Home
Walsh, Frank S.; Dickson, George
doi: 10.1002/bies.950110402pmid: 2695078
The neural cell adhesion molecule (N‐CAM) is believed to be a key regulator of adhesive events in the nervous system and skeletal muscle. The recent isolation of N‐CAM cDNAs from different tissues has identified a high degree of diversity in primary amino acid sequence between different isoforms. In this article, we review these recent studies and discuss methods for unravelling the functional consequences of the generation of multiple N‐CAM polypeptides using gene transfection approaches.
doi: 10.1002/bies.950110403pmid: 2695079
The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins, named catenins, which are structurally related in different cell types of various species. This complex formation connects uvnomorulin and cytoskeletal structures and might, moreover, be involved in other adhesion‐dependent mechanisms.
doi: 10.1002/bies.950110404pmid: 2695080
Baculovirus vectors have proven useful in producing high levels of biologically active eukaryotic proteins and providing cellular fractions which are enriched in the protein of interest. Expression occurs in infected insect cells which also provide a suitable environment for posttranslational modification and folding of the protein product. Stable baculovirus vectors can be constructed rapidly with a minimum of viral manipulation.
Knust, Elisabeth; Campos‐Ortega, José A.
doi: 10.1002/bies.950110405pmid: 2515857
The extent of neurogenesis in Drosophila is under the control of the so‐called neurogenic genes, named for their mutant phenotype of causing neural hyperplasia. Their wild‐type products appear to be responsible for a signal chain that decides the fate of ectodermal cells in the embryo. Various kinds of data, from cell transplantation experiments as well as from genetic and molecular analyses, suggest that the proteins encoded by the genes Notch and Delta may act at the membrane of the signal‐transmitting cells to provide a ligand to a still unknown receptor molecule; in contrast, the locus of Enhancer of split codes for several functions related to the transduction and further processing of the signal.
Wyatt, Jacqueline R.; Puglisi, Joseph D.; Tinoco, Ignacio
doi: 10.1002/bies.950110406pmid: 2695075
The three‐dimensional structures adopted by RNA molecules are crucial to their biological functions. The nucleotides of an RNA molecule interact to form characteristic secondary‐structure mctifs. Tertiary interactions orient these secondary‐structure elements with respect to each other to form the functional RNA. Here we describe the basic structural elements with special emphasis on a novel tertiary motif, the pseudoknot.
Showing 1 to 10 of 12 Articles